不同时间缺氧对脂肪细胞炎症因子表达的影响

Effect of different incubation time of hypoxia on the levels of inflammatory cytokines in adipocytes

目的:探讨不同时间缺氧孵育后脂肪细胞炎症因子TNF-α和MCP-1表达的变化。方法:常氧(21%O2)和缺氧(1%O2)4、8、16 h孵育脂肪细胞,免疫印迹法检测葡萄糖转运子1(GLUT1)的表达,Real-time PCR法测定脂肪细胞TNF-α和MCP-1基因表达。结果:与常氧组相比,缺氧孵育的脂肪细胞GLUT1蛋白和TNF-α、MCP-1基因的表达均升高(P=0.002、P=0.016),并且随着缺氧时间的延长而显著升高。结论:缺氧造成脂肪细胞GLUT1和TNF-α、MCP-1的表达升高,缺氧时间越长,升高越明显。

Objective： To explore the effect of different incubation time of hypoxia on expression of inflammatory cytokines TNF-α and MCP-1 in adipocytes.Methods： 3T3-L1 adipocytes were treated with normoxia（21% O 2） or hypoxia（1% O 2） conditions for 4,8,or 16 hours.The glucose transporter 1（GLUT1） was detected by Western blot.The mRNA expressions of TNF-α and MCP-1 by adipocytes were determined by real-time PCR.Results： The protein expression of GLUT1 and the mRNA expressions of TNF-α and MCP-1 were higher in hypoxia-treated 3T3-L1 adipocytes than nomoxia-treated cells（P=0.002,P=0.016）.Moreover,the expression levels were increased with prolonged incubation time of hypoxia.Conclusion： The mRNA expressions of TNF-α and MCP-1,as well as protein expression of GLUT1 by adipocyte are increased under hypoxia treatment,and the longer incubation time of hypoxia,the more obviously level increases.