巨噬细胞表型偏移相关标志物在大鼠心肌梗死区的动态表达

Dynamic expression profile of macrophage polarization related biomarkers following myocardial infarction

目的:探讨急性心肌梗死(AMI)后梗死区巨噬细胞表型偏移相关标志物的动态变化规律。方法:Wistar大鼠随机分为心肌梗死组和假手术组。心肌梗死组采用开胸并结扎左冠状动脉前降支的方法建立AMI模型。假手术组仅开胸并穿结扎线,不结扎前降支。在心肌梗死模型建立后6 h、24 h以及6 d分别处死一批大鼠(每组每个时间点3～4只)。实时定量PCR法分别测定梗死区巨噬细胞M1偏移的2个标志物[趋化因子配体-2(Ccl2)和趋化因子配体-5(Ccl5)]及M2偏移的2个标志物[精氨酸酶-1(Arg-1)和白介素-10(IL-10)]的mRNA表达水平。结果:与假手术组相比,心肌梗死组梗死区心肌组织M1偏移的标志物Ccl2表达水平在6 h升高,24 h达到高峰,分别是假手术组的2.3倍和4.29倍(P<0.05)。Ccl5表达量在24 h升高达到假手术组的2倍(P<0.05)。M2偏移的标志物Arg-1和IL-10的表达水平在24 h和6 d明显升高。Arg-1在24 h较假手术组升高了14.5倍,并持续升高至6 d(P<0.05);IL-10在24 h和6 d分别较假手术组升高了10.3倍和2.9倍(P<0.05)。结论:AMI发生后,浸润于梗死区的巨噬细胞表型呈动态偏移,由促炎症表型(M1偏移)向促修复表型(M2偏移)转化。

Objective： To study the dynamic expression profile of macrophage polarization related biomarkers following acute myocardial infarction（AMI）.Methods： Male Wistar rats were randomized into AMI group and sham group.AMI group was subjected ligation of coronary artery.Sham group was not underwent occlusion of coronary artery.3-4 rats were sacrificed in 6 h,24 h and 6 d following myocardial infarction.Real-time PCR was used to analyze the quantity of M1 macrophage related biomarker（Ccl2,Ccl5） and M2 macrophage related biomarker（Arg-1,IL-10） mRNA.Results： Following AMI,a significant up-regulation of M1 macrophage related biomarker Ccl2 and Ccl5 were revealed by Real-time PCR at 6 h and 24 h post infarction（P0.05）.M2 macrophage related biomarker Arg-1 and IL-10 mRNA expression increased from 24 h to 6 d（P0.05）.Conclusion： This study demonstrates that a dynamic switch of macrophage phenotype polarization related markers from M1 to M2 following myocardial infarction.