摘要
目的:探讨急性心肌梗死(AMI)后梗死区巨噬细胞表型偏移相关标志物的动态变化规律。方法:Wistar大鼠随机分为心肌梗死组和假手术组。心肌梗死组采用开胸并结扎左冠状动脉前降支的方法建立AMI模型。假手术组仅开胸并穿结扎线,不结扎前降支。在心肌梗死模型建立后6 h、24 h以及6 d分别处死一批大鼠(每组每个时间点3~4只)。实时定量PCR法分别测定梗死区巨噬细胞M1偏移的2个标志物[趋化因子配体-2(Ccl2)和趋化因子配体-5(Ccl5)]及M2偏移的2个标志物[精氨酸酶-1(Arg-1)和白介素-10(IL-10)]的mRNA表达水平。结果:与假手术组相比,心肌梗死组梗死区心肌组织M1偏移的标志物Ccl2表达水平在6 h升高,24 h达到高峰,分别是假手术组的2.3倍和4.29倍(P<0.05)。Ccl5表达量在24 h升高达到假手术组的2倍(P<0.05)。M2偏移的标志物Arg-1和IL-10的表达水平在24 h和6 d明显升高。Arg-1在24 h较假手术组升高了14.5倍,并持续升高至6 d(P<0.05);IL-10在24 h和6 d分别较假手术组升高了10.3倍和2.9倍(P<0.05)。结论:AMI发生后,浸润于梗死区的巨噬细胞表型呈动态偏移,由促炎症表型(M1偏移)向促修复表型(M2偏移)转化。
Objective: To study the dynamic expression profile of macrophage polarization related biomarkers following acute myocardial infarction(AMI).Methods: Male Wistar rats were randomized into AMI group and sham group.AMI group was subjected ligation of coronary artery.Sham group was not underwent occlusion of coronary artery.3-4 rats were sacrificed in 6 h,24 h and 6 d following myocardial infarction.Real-time PCR was used to analyze the quantity of M1 macrophage related biomarker(Ccl2,Ccl5) and M2 macrophage related biomarker(Arg-1,IL-10) mRNA.Results: Following AMI,a significant up-regulation of M1 macrophage related biomarker Ccl2 and Ccl5 were revealed by Real-time PCR at 6 h and 24 h post infarction(P0.05).M2 macrophage related biomarker Arg-1 and IL-10 mRNA expression increased from 24 h to 6 d(P0.05).Conclusion: This study demonstrates that a dynamic switch of macrophage phenotype polarization related markers from M1 to M2 following myocardial infarction.
出处
《天津医科大学学报》
2012年第1期14-16,共3页
Journal of Tianjin Medical University
基金
国家自然科学基金资助项目(81070121)
关键词
巨噬细胞
急性心肌梗死
表型偏移
大鼠
macrophages
acute myocardial infarction
phenotype polarization
rat