半夏花粉活力检测及杂交研究

Study on Pollen Viability and Hybridization of Pinellia ternate

为建立半夏杂交育种体系,在利用优化的半夏花粉活力检测体系对江苏海门、山西新绛、江苏泰州、重庆武陵等4个半夏居群的花粉活力日变化情况进行测定的基础上,对各居群间配合力进行了检测。结果显示,4个居群中,以山西新绛半夏的花粉活力最高,其次为江苏泰州半夏,江苏海门半夏及重庆武陵半夏的花粉活力较低;在同一天中,各居群半夏花粉活力在早晨最高;不同居群半夏间杂交亲和力差异显著。上述结果表明,各居群间花粉和柱头亲和性是影响杂交结实率的主要因素。

To establish a breeding system and provide information for the genetic modification of Pinellia ternata,the combining ability as well as the daily change in pollen viability of 4 Pinellia ternate populations which were collected from Haimen city of Jiangsu province,Xinjiang city of Shanxi province,Taizhou city of Jiangsu province and Wuling city of Chongqing were tested.The results showed that the pollen viabilities of all tested 4 populations were the highest in the morning,and the population collected from Xinjiang city of Shanxi province had the highest pollen viability,followed by that from Taizhou city of Jiangsu province,and the pollen viabilities of Pinellia ternate from Haimen city of Jiangsu province and Wuling city of Chongqing were lower.The hybrid compatibility between different populations was obviously different.Based on the above results,the compatibility of pollen and stigma between various populations was the main factor affecting the hybrid setting rate of Pinellia ternata.