民猪LPL基因真核表达载体的构建

Construction of eukaryotic cell expression vector of LPL gene in Min pig

为了进一步分析脂蛋白脂酶(Lipoprotein lipase,LPL)基因的生物学功能。采用克隆测序的方法获得了民猪LPL基因的完整CDS序列,将其克隆到pMD-18T载体上,将测序验证正确的基因序列插入到pcDNA3.1(+)质粒载体中,构建了pcDNA-LPL真核表达载体。结果表明,所得序列与GenBank中登录的猪LPL基因同源性为99.30%,说明LPL基因的真核载体构建成功。该研究为在细胞水平上研究LPL的表达和功能奠定了试验基础,为进一步分析LPL基因功能提供了理论依据。

To further analyze the lipoprotein lipase（Lipoprotein lipase,LPL） gene biological functions.In this study,cloning and sequencing methods were used to obtain the complete CDS sequence of the Min pig LPL gene,then PCR product was cloned into pMD-18T vector and sequenced to verify the correct gene sequence inserted into the pcDNA3.1（＋） plasmid vector to construct the pcDNA-LPL eukaryotic expression vector.The results showed that the obtainded sequence with GenBank pig LPL gene was 99.30% homology,indicating that LPL eukaryotic vector was successfully constructed.This study provided a experimetal basis for research the expression and function of LPL at the cellular level and also provided a theoretical basis for further analysis of LPL gene function.