木犀草素对冷保存大鼠心脏心功能及氧化应激反应的影响

Effects of Luteolin on Isolated Heart Function and Oxidative Stress Reaction after Hypothermic Preservation

目的研究木犀草素对冷保存大鼠心脏心功能及氧化应激反应的影响.方法将40只成年SD大鼠随机分成2组(n=20):对照组(心脏保存液为UW液)及实验组(心脏保存液为含30μmol/L木犀草素的UW液).利用Langendorff离体心脏灌流法,观察2组液体保存心脏18 h并复灌60 min后心脏的收缩与舒张功能,冠脉流出液中磷酸肌酸激酶(CK)、乳酸脱氢酶(LDH)的释放量及心肌组织中超氧化物歧化酶(SOD)、丙二醛(MDA)的含量变化.结果实验组复灌期心脏的收缩功能(LVPSP,+dp/dtmax)、舒张功能(-dp/dtmax)及心率显著高于对照组(P<0.05),冠脉流出液中CK、LDH的漏出量和心肌组织中MDA含量明显低于对照组(P<0.05),而心肌组织中SOD活力明显高于对照组(P<0.05).结论木犀草素能显著改善冷保存后心脏的心功能,减少心肌氧化损伤.

Objective To study effects of luteolin on isolated heart function and oxidative stress reaction after hypothermic preservation.Methods Forty male SD rats were randomly divided into 2 groups（n=20 in each group）：control group（heart preservative solution was UW solution） and experiment group（added 30 μmol/L luteolin in UW solution）.Langendorff model of isolated rat heart was used.The cardiac contractile function and diastolic function were determined 18 hours after hypothermic preservation followed by 60 min reperfusion.The amount of creatine kinase（CK）and lactic dehydrogenase（LDH）leakage in the coronary effluent,the content of Superoxide Dismutase（SOD）and malondialdehyde（MDA）in myocardial tissue were measured.Results Luteolin significantly improved the recovery of cardiac contractile function（LVPSP,＋dp/dtmax）and diastolic function（-dp/dtmax）after hypothermic preservation,reduced the leakage of CK and LDH in the coronary effluent,reduced the content of MDA in myocardial tissue and increase the content of SOD in myocardial tissue,compared with control group.Conclusion Luteolin can significantly improve isolated heart function and reduce oxidative stress reaction in myocardial tissue after hypothermic preservation.