大鼠脊髓损伤亚急性期NSCs脊髓内移植对脊髓神经电生理的影响

Effects of Neural Stem Cells Transplantation on Spinal Nerves Electrophysiology in Subacute Stage

目的研究神经脊髓损伤的亚急性期(第7天)神经干细胞(neural stem cells,NSCs)脊髓内移植对大鼠受损伤脊髓神经电生理的影响.方法正常成年SD雌性大鼠15只,分为3组:单纯全横断组、假手术组和神经干细胞移植组.测定3组大鼠的皮层体感诱发电位(cortical somatosensoryevoked potential,CSEP)、运动诱发电位(motion evoked potential,MEP)和电针刺激大脑皮质运动区.结果神经干细胞移植组的诱发电位CSEP、MEP的潜伏期短于单纯脊髓全横断组大鼠,电针刺激大脑皮质运动区发现NSC移植组大鼠双后肢出现明显收缩,且以对侧更为明显.结论神经干细胞脊髓内移植后能部分促进损伤脊髓的感觉和运动传导功能的恢复.

Objective To study the effects of neural stem cells transplantation on spinal nerves electrophysiology in subacute stage.Methods 15 femal adult rats were divided into three groups：sham operation group,merely spinal cord transsection group,and NSC transplantation group.The cortical somatosensory evoked potential（CSEP）and Motion Evoked Potential（MEP）were evaluated and the cortical motor area stimulation by electro-acupuncture was performed in the three groups.Results Latency of CSEP and MEP of rats in NSC transplantation group was shorter than merely spinal cord transsection group.Neural stem cells transplantation led to hind limbs contraction after cortical motor area stimulation by electro-acupuncture.Conclusion Neural stem cells transplantation can partially promotes the recovery of sensory nerve and motor nerve conduction of injured spinal cord.