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厚壳贻贝血细胞颗粒的蛋白质组学分析 被引量：4

Proteomic analysis of granule of hemocytes from Mytilus coruscus
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摘要 贝类的血细胞在宿主的免疫防御机制中发挥着重要的作用。文章利用线性离子阱-四级杆质谱对厚壳贻贝(Mytilus coruscus)血细胞的颗粒蛋白质组分进行了Shotgun分析,结合Mascot数据库搜索,共鉴定了125种高丰度蛋白,其中包括细胞骨架蛋白、细胞粘附蛋白、精氨酸激酶和代谢相关酶类等。此结果为深入了解厚壳贻贝血细胞颗粒的蛋白组成、贻贝免疫分子标记的筛选及其免疫机制奠定了基础。 The blood cells of mussel play an important role in the host＇s immune defense mechanism. We explore the protein composi- tion in the granule of hemocytes from Mytilus coruscus by using liuear-ion-trap-quadrupole mass spectrometry （LTQ-Orbitrap） combined with Shotgun strategy. A total of 125 high-abundance proteins are identified by Mascot searching methods, including cytoskeletal pro- teins, cell adhesion proteins, arginine kinases and metabolism-related enzymes, etc.. The results lay a foundation for further studies on the composition of mussel protein particles, screening of its immune molecular markers as well as its immune mechanism.
作者 王信超 孙敬敬 范美华 武梅 石戈 王日昕 廖智
机构地区 浙江海洋学院海洋科学学院
出处 《南方水产科学》 CAS 北大核心 2012年第2期7-14,共8页 South China Fisheries Science
基金 浙江省科技厅面上科研农业项目(2008C22026 2009C32016)
关键词 厚壳贻贝 血细胞颗粒 LTQ—Orbitrap 质谱 SHOTGUN Mytilus coruscus hemocytes granule LTQ-Orbitrap mass spectrometry Shotgun
分类号 S917.4 [农业科学—水产科学]
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参考文献28

  • 1SUN Jingfeng,WU Xinzhong.Histology,ultrastructure and mor-phogenesis of a Rickettsi-like organism causing disease in the oys-ter,Crassostrea ariakensis[J].Invertebr Pathol,2004,86(3):77-86.
  • 2CHARLET M,CHERNYSH S,PHILIPPE H,et al.Isolation ofseveral cysteine-rich antimicrobial peptides from the blood of a mol-lusk,Mytilus edulis[J].J Biol Chem,1996,271(36):21808-21813.
  • 3MITTA G,VANDENBULCKE F,HUBERT F,et al.Involvementof Mytilins in mussel antimicrobial defense[J].J Biol Chem,2000,275(17):12954-12962.
  • 4YANG Y S,MITTA G,CHAVANIEU A.Solution structure andactivity of the synthetic four-disulfide bond Mediterranean musseldefensin(MGD-1)[J].Biochemistry,2000,39(47):14436-14447.
  • 5MITTA G,HUBERT F,DYRYNDA E A,et al.Mytilin B andMGD-2,two antimicrobial peptides of marine mussels:gene struc-ture and expression analysis[J].Dev Comp Immunol,2000,24(4):381-393.
  • 6MITTA G,HUBERT F,NOEL T,et al.Myticin,a novel cyste-ine-rich antimicrobial peptide isolated from hemocytes and plasma ofthe mussel Mytilus galloprovincialis[J].Eur J Biochem,1999,265(1):71-78.
  • 7FRIEBELB,RENWRANTZL.Application ofdensitygradientcen-trifugation for separation of eosinophilic and basophilic haemocytesfrom Mytilus edulis and characterization of both cell groups[J].Comp Biochem Physiol,1996,112(1):81-90.
  • 8BAYNE C J,MOORE M N,CAREFOOT T H,et al.Hemolymphfunctions in Mytilus californianus:the cytochemistry of hemocytesand their responses to foreign implants and hemolymph factors inphagocytosis[J].Invertebr Pathol,1979,34(1):1-20.
  • 9CHENG T C.Bivalves invertebrate blood cells[M].New York:Academic Press,1981:233-330.
  • 10MOORE M N,LOWE D M.The cytology and cytochemistry of thehemocytes of Mytilus edulis and their response to experimentally in-jected carbon particles[J].Invertebr Pathol,1977,29(1):18-30.

二级参考文献64

  • 1Cellura C, Toubiana M, Parrinello Net al, 2007. Specific expression of antimicrobial peptide and HSP70 genes in response to heat-shock and several bacterial challenges in mussels. Fish Shellfish Immunol, 22(4): 340-350.
  • 2Charlet M, Chernysh S, Philippe H et al, 1996. Innate immunity. Isolation of several cysteine-rich antimicrobial peptides from the blood of a mollusk, Mytilus edulis. J Biol Chem, 271:21808-21813.
  • 3Hubert F, Noel T, Roch P, 1996. A member of the arthropod defensin family from edible Mediterranean mussels (Mytilus galloprovincialis). Eur J Biochem, 240:302-306.
  • 4Karimi M, Inze D, Depicker A, 2002. GATEWAY vectors for Agrobacterium-mediated plant transformation. Trends Plant Sci, 7(5): 193-195.
  • 5Mcphee J B, Hancock R E W, 2005. Function and therapeutic potential of host defence peptides. J Peptide Sci, 11: 677-687.
  • 6Mitta G, Hubert F, Dyrynda E A et al, 2000. Mytilin B and MGD2, two antimicrobial peptides of marine mussels: gene structure and expression analysis. Dev Comp Immunol, 24(4): 381-393.
  • 7Mitta G, Hubert F, NoeEl T et al, 1999a. Myticin, a novel cysteine-rich antimicrobial peptide isolated from hemocytes and plasma of the mussel Mytilus galloprovincialis. Eur J Biochem, 265:71-78.
  • 8Mitta G, Vandenbulcke F, Hubert F et al, 1999b. Mussel defensins are synthesised and processed in granulocytes then released into the plasma after bacterial challenge. J Cell Sci, 112(23): 4233-4242.
  • 9Mitta G, Vandenbulcke F, Hubert F et al, 2000. Involvement of mytilins in mussel antimicrobial defense. J Biol Chem, 275(17): 12954-12962.
  • 10Piano A, Valbonesi P, Fabbri E, 2004. Expression of cytoprotective proteins, heat shock protein 70 and metallothioneins, in tissues of Ostrea edulis exposed to heat and heavy metals. Cell Stress Chaperones, 9(2): 134-142.

共引文献46

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南方水产科学
2012年 第2期

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