基于能量代谢酶相对活力评价大菱鲆卵质

Quality assessment of oosperm of turbot(Scophthalmus maximus) based on energy metabolism-related relative enzyme activities

采用卵径形态比较和能量代谢关键酶生理生化分析方法,对大菱鲆(Scophthalmus maximus)卵质进行了评价。结果显示,大菱鲆受精卵卵径平均值为(1.053±0.020)mm,不同批次的受精卵卵径间无显著差异。双变量相关统计分析结果显示,大菱鲆卵径与悬浮率、受精率和孵化率间相关性皆不显著。基于能量代谢关键酶统计结果显示,大菱鲆受精卵孵化率与参与蛋白代谢关键酶谷草转氨酶(AST)相对活力存在显著相关性[EAST≥(15.159±3.300)U.mg-1,HHatchingrate>20%,Spearman相关系数为R=0.675,P=0.003],悬浮率和受精率与AST显著相关;大菱鲆受精卵悬浮率、受精率和孵化率与参与糖代谢关键酶丙酮酸激酶(PK)间相关性均不显著。该研究表明,AST相对活力可以作为用于评价大菱鲆卵质的生理生化参数,蛋白质在大菱鲆受精卵能量代谢过程中是一个重要的能量来源。

We assessed the oosperm quality of turbot （ Scophthalmus maximus） based on the morphological comparison of oosperm di- ameter and energy metabolism-related relative enzyme activities. The results show that the average oosperm diameter for turbot is （ 1. 053 ±0.020 ） mm and no significant difference is found among different batches. Bivariate correlation statistics reveal that the cor- relation is insignificant among oosperm diameter and suspensibility as well as fertilization rate and hatching rate. The statistics of energy metabolism-related relative enzyme activities indicate that significant correlation is found between hatching rate and aspartate amino