依达拉奉在星形胶质细胞持续缺糖缺氧损伤中的作用

Effect of Edaravone on the Damage of Astrocytes Induced by Continuous Oxygen-glucose Deprivation

【目的】研究自由基清除剂依达拉奉对持续缺糖缺氧诱导的星形胶质细胞（AS）损伤的影响。【方法】用缺糖缺氧4h处理大鼠原代AS星形胶质细胞作为As持续缺血的体外模型，实验分为4组：①空白对照组，②依达拉奉对照组（300uM），③缺糖缺氧组，④依达拉奉（300uM）＋缺糖缺氧组。测定各组细胞乳酸脱氢酶（LDH）释放量，观察并测定细胞内活性氧簇（ROS）含量、线粒体膜电位（MMP）水平。【结果】AS经持续缺糖缺氧损伤后，与空白对照组相比，细胞LHD释放量及ROS含量显著增加（P〈0．05），MMP显著下降（P〈0．05）；依达拉奉＋缺糖缺氧组与缺糖缺氧组相比，细胞LDH释放量及R0s含量显著下降（P〈0．05），MMP显著升高（P〈0．05）；依迭拉奉对照组与空白对照组相比3项指标无显著差异（P〉0．05）。【结论】依达拉奉通过减轻氧化应激、保护线粒体功能，减轻持续缺糖缺氧诱导的As。

[Objective] To investigate the effect of free radical scavenger edaravone on the damage of astrocytes（AS） induced by continuous oxygen-glucose deprivation（OGD）. [Methods]The primary-cultured rat astrocytes treated by OGD were selected as the models of continuous ischemia in vitro and divided into control group, edaravone （300uM） group,OGD group and edaravone（300uM）＋OGD group. Lactate dehydrogenase（LDH） release of the cells was measured. The reactive oxygen species（ROS） and mitochondrial membrane potential（MMP） were observed and detected. [Results] Compared with the control group, LHD release and ROS content markedly increased, and MMP markedly decreased after the damage of AS induced by OGD（ P〈0.05）. Compared with OGD group, LDH release and ROS content in edaravone＋ OGD group decreased significantly（ P〈0.05）, while MMP increased significantly（ P〈0.05）. There was no significant difference in above indicators between edaravone group and control group（ P 〉0.05）. [Conclusion] Edaravone can alleviate the AS damage induced by continuous OGD through ameliorating oxidative stress and protecting mitochondria function.