摘要
【目的】研究重型和危重型手足口病患儿免疫功能的变化。【方法】以本院154例危重型和96例重型手足口病患儿及96例健康儿童为研究对象;采用散射比浊法检测其血清免疫球蛋白和补体,采用流式细胞仪检测法检测患儿外周血T、B细胞亚群和NK细胞。【结果】重型、危重型手足口病患儿的B细胞、IgG、IgM、C3和C4水平明显升高,差异有统计学意义(P〈0.05),而NK细胞、IgA和IgE水平无明显变化(P〉0.05),重型、危重型手足口病患儿的总T细胞、CD4+细胞和cD4+/cD8+水平下降,差异有统计学意义(P〈0.05);重型手足口病患儿的CD8+细胞水平明显升高,差异有统计学意义(P〈0.05),而危重型手足口病患儿的CD8+细胞水平无变化(P〉0.05)。【结论】重型和危重型手足口病患儿体内的NK细胞无变化,体液免疫功能和C3、C4水平升高,其主要是导致患儿抗感染的细胞免疫应答无法有效诱导,从而使机体无法有效地清除病毒。
[Objective]To explore the change of immunologic function in children with critical and severe hand-foot- mouth disease(HFMD). [Methods] Totally 154 children with critical HFMD, 96 children with severe HFMD and 96 healthy children in our hospital were enrolled in this study. Serum immunoglobulin and complements were measured by u- sing nephelometry. Flow cytometry was used to detect T, B cell subsets and NK cells in peripheral blood. [Results]The levels of B cells, IgG, IgM, C3 and CA in children with critical and severe HFMD increased obviously, and there was sig- nificant difference( P %0.05), but the levels of NK cells, IgA and IgE had no obvious change( P〉0. 05). The total T cells, CEN+ cells and CI)4+/CD8+ cells in children with critical and severe HFMD decreased, and there was significant difference( P 〈0.05). The level of CD8+ cells in children with severe HFMD obviously increased, and there was signifi- cant difference ( P〈0. 05 ). The level of CD8+ cells in children with critical HFMD had no change ( P〈0. 05 ). [Conclusion]The NK cells in children with critical and severe HFMD has no change, but the function of humoral immuni- ty and the levels of C3 and C4 increase, which results in that cellular immunologic response can not be effectively induced so that the body can not effectively remove the virus.
出处
《医学临床研究》
CAS
2012年第3期511-513,共3页
Journal of Clinical Research
关键词
手足口病/免疫学
Hand,foot and mouth disease/IM