EtNBSe联合光动力疗法对成纤维细胞增殖及TGF-β_1分泌的影响

The of Influence EtNBSe Together with Photodynamic Therapy on the Fibroblast Proliferation and TGF-β_1 Secretion

目的:观察EtNBSe(5-ethylamino-9-diethylamino benzo[a]phenoselenazinium chloride)联合光动力疗法(photodynamic therapy,PDT)对人包皮成纤维细胞体外增殖及分泌表型的影响。方法:成纤维细胞接种至培养板,采用不同浓度的EtNBSe处理细胞2h后,给予同一时间剂量的红光辐照,MTT法检测细胞的功能;酶联免疫吸附试验(ELISA)法检测转化生长因子-β1(transforming growth factor-β1,TGF-β1)水平的变化。结果:(1)与空白组比较,细胞接受EtNBSe-PDT处理后,EtNBSe(60、120pM)组有增殖活性(P<0.05);(2)与空白组比较,EtNBSe(60、120pM)组,TGF-β1浓度分别增加28.57%和45.11%(P<0.05)。结论:EtNBSe-PDT可促进体外生长的成纤维细胞增殖活性,诱导成纤维细胞TGF-β1的分泌。

Objective：To investigate the influences of 5-ethylamino-9-diethylamino benzo[a]phenoselenazinium chloride （EtNBSe） together with Photodynamic Therapy on human foreskin fibroblast cells proliferation in vitro and secretion phenotype.Methods：Inoculate fibroblasts to the culture plates, treated with different concentrations of EtNBSe, and given the same time red light exposure 2 hours after treatment. Using MTT assay examine the cell functions, and ELISA assay examine the changes of TGF-β1.Results：Compared with the control group, EtNBSe-PDT treated cells especially EtNBSe（60 or 120pM） group proliferated obviously（P0.05）; and the concentration of TGF-β1 in these two groups was increased 28.57% and 45.11% respectively（P0.05）.Conclusion：In vitro, EtNBSe-PDT treatments promote the activity of growing fibroblast proliferation, induce TGF-β1 secretion.