发酵产云芝胞外多糖的分析及其在卷烟中的应用

Analysis of exopolysaccharides from fermented Coriolus versicolor and its application on tobacco flavour

对发酵产云芝胞外多糖进行提取和脱蛋白处理得到精制云芝胞外多糖,之后过SepharoseCL-6B凝胶柱得到一个多糖组分,然后用红外光谱技术对该多糖组分的结构进行表征得知其为酸性多糖;将精制云芝胞外多糖以不同的浓度注入卷烟中,利用吸烟机捕集烟气粒相物计算出云芝胞外多糖在烟气中的转移率在2.76%～4.78%之间变动;用单料烟丝进行加香评吸实验,结果表明在精制胞外多糖添加量为0.04%时明显地改善了卷烟的吸食品质,起到掩盖杂气,去除刺激,改善余味的作用,使香气细腻程度有所提升。这为发酵产云芝胞外多糖在卷烟中的应用提供了一定的理论支持。

Exopolysaccharides （EPS）were extracted by ethanol from fermented fungi Coriolus versicolor, and purified by gel filtration chromatography on Sepharose CL-6B. The preliminary characterization of EPS was elucidated by IR, and EPS was an acidic carbohydrate. Different concentrations of EPS from Coriolus versicolor were injected in cigarettes,and calculated the transfer rates.It was showed that the transfer rate of EPS in gas was in the rage of 2.76%-4.78% and the adding 0.04% EPS could improve the quality,cover the smoking foreign gas, remove stimulus, and improve aroma aftertaste, fruity feeling and coordination. The results provided a strong support for the application of EPS in cigarette industry.