响应面法分析优化米曲霉产β-葡萄糖苷酶液体发酵培养基

Optimization and analysis of β-glucosidase production by Aspergillus oryzae in liquid cultures using response surface methodology

利用快速有效的响应面分析法对米曲霉ASPE0485产β-葡萄糖苷酶的发酵培养基进行优化,利用PlackettBurman显著因子实验和Box-Behnken响应面分析优化了β-葡萄糖苷酶产生菌的发酵培养基,确定摇瓶发酵的最佳培养基组成为(%,w/v):玉米芯3.8%、大豆蛋白胨0.5%、KH2PO40.5%、MgSO4.7H2O0.05%、CaCl20.05%、吐温-800.27%和接种量5.3%;在此条件下发酵,得到酶活为21.1U/mL比原始酶活(17.65U/mL)提高了19.55%。

Response surface analysis was conducted on Aspergillus oryzae ASPE0485 to determine the optimal culture medium for β-glucosidase fermentation.The Box-Behnken and Plackett-Burman design was utilized to the response surface analysis to determine the optimal culture medium consistents（ %, w/v）were as follow： corncob 3.8% ,soya peptone 0.5% ,KH2PO4 0.5% ,MgSO4 . 7H2O 0.05% ,CaCI2 0.05% ,tween-80 0.27% and inoculum size 5.3%.The β-glucosidase activity was 21.1U/mL under the optimal conditions, which was more 19.55% than the original strain activity（17.65U/mL）.