甘薯羽状斑驳病毒外壳蛋白基因的分子变异

Molecular variation of Sweet potato feathery mottle virus coat protein gene

应用单链构象多态性(single-strand conformation polymorphism,SSCP)技术结合核苷酸序列测定的方法,对我国甘薯主产区11个省份的甘薯羽状斑驳病毒(Sweet potato feathery mottle virus,SPFMV)外壳蛋白(CP)基因的分子变异情况进行了研究。结果表明,SPFMV CP基因的RT-PCR产物表现了较丰富的图谱类型,50个分离物共产生9种主要的SSCP带型;对显示不同带型的20个样品的CP基因进行了序列测定和进化树分析,CP基因核苷酸序列一致性为77.2%～99.9%。说明这些样品的SPFMV的CP基因存在较大的分子变异,可划分为EA、RC、O和C 4个株系。

Virus samples were collected from 11 provinces in China,and the molecular variation of Sweet potato feathery mottle virus（SPFMV）coat protein（CP） gene were analyzed using single-strand conformation polymorphism（SSCP） and sequence analysis.SSCP analysis of 50 isolates showed that these isolates produced 9 kinds of SSCP band forms.The results of sequence comparison and phylogenetic analysis showed that the identities of the nucleotide sequences of CP genes among these isolates were 77.2%-99.9%.SPFMV CP genes of different isolates in China had great molecular variability and could be divided into four diverged groups at least,including the East Africa（EA）,Russet Crack（RC）,Ordinary（O） and Common（C） strain groups.