不同来源间充质干细胞治疗小鼠放射性肺损伤的研究

Comparison of Mesenchymal Stem Cells Derived from Different Tis sues on Moderating Irradiation Induced Lung Injury

目的:观察小鼠骨髓、肺及胚胎背主动脉区来源的间充质干细胞(MSC)对小鼠肺损伤的治疗作用。方法:将小鼠随机分为5组,即正常组、单纯照射组、OP9细胞治疗组、胚肺MSC治疗组、胚胎背主动脉区来源MSC治疗组(DA组),分别将OP9细胞、胚肺和DA区MSC移植入受体小鼠,4、9、13、17、23周后观察各组小鼠外观、肺组织的病理变化。结果:小鼠外观评分均值由高到低顺序为正常组、DA组、胚肺MSC组、OP9组、单纯照射组,正常组明显好于各组(均为P<0.01),单纯照射组明显差于DA组、胚肺MSC组(均为P<0.01);DA组、胚肺MSC组及OP9组的两两配对t检验均为P>0.05。肺病理镜检肺损伤评分均值由高到低顺序为正常组、胚肺MSC组、OP9组、DA组、单纯照射组。肺病理图像分析肺泡情况由好到差顺序为正常组、胚肺MSC组、DA组、OP9组、单纯照射组。外观评分与病理镜检的相关性检验P=0.040,与图像分析的相关性检验P=0.039。结论:不同来源的MSC均有减轻放射性肺损伤的作用,其作用强度相似。

Objective： To compare the role of mesenchymal stem cells （MSC） derived from mouse embryonic lung, bone marrow and embryonic dorsal aorta（DA） area in the treatment of irradiation induced mouse model of lung injury. Methods： The mice were divided randomly into five groups： normal control group（A）, irradiation with- out MSC group（B）, embryonic lung MSC treatment group（C）, OP9 treatment group（D） and DA MSC treatment group（E）. Four, nine, thirteen, seventeen and twenty-three weeks after MSC engraftment, the change in appear- ance of the mice in each group was observed, and then their lungs were harvested after the mice were sacrificed. The pathology of the lung structure was observed by HE stalling. Results： The mean score of mice appearance was the highest in group A and the lowest in group B. There were no significant difference in the mean appear- ance score between group C, D and E. The mean alveolitis score was related to the mean appearance score, P= 0.040.The image analysis of alveoli area was related to the mean appearance score, P=0.039. Conclusion： The ex- tent of irradiation induced lung injury could be moderated through the treatment of MSC derived from embryonic lung, OP9 and embryonic dorsal aorta area. The treatment of MSC derived from different tissue is similar.