摘要
目的:建立检测田间转基因抗草甘膦油菜的方法。方法:用CTAB法提取DNA,经PCR分别扩增内参基因BnACCg8,及抗草甘膦外源基因CP4-EPSPS、FMV 35S启动子和E9 3'终止子等4种基因的片段,以琼脂糖凝胶电泳对扩增产物进行分析比对。结果:抗草甘膦油菜中分别扩增出与外源基因FMV 35S启动子、E9 3'终止子和CP4-EPSPS大小一致的片段。结论:该方法能有效地用于转基因抗草甘膦油菜的检测。
Objective： To develop a method for detection of transgenic glyphosate resistant rape. Methods： DNA of glyphosate resistant rape was extracted by the method of CTAB, then reference gene BnACCg8, and three exoge nous genes of FMV 35S promoter, E9 3 terminator and CP4EPSPS were amplified by PCR, and the amplified products were detected and analyzed on agar sugar gel electrophoresis. Results： There were three fragments which as same size as three exogenous genes of FMV 35S promoter, E9 3 terminator and the CP4EPSPS respectively in glyphosate resistant rape. Conclusion： This method can be applied in determination of transgenie glyphosate re sistant rape.
出处
《生物技术通讯》
CAS
2012年第2期242-244,共3页
Letters in Biotechnology
关键词
抗草甘膦
转基因
油菜
PCR检测
glyphosate
genetically modified
rapeseed
PCR detection
