消痰和胃方防治化疗延迟性呕吐的机制研究

Mechanism of antiemetic effect of Xiaotan Hewei Decoction on chemotherapy-induced delay emesis

目的:探索消痰和胃方防治化疗诱导延迟性呕吐的作用机制。方法:大鼠分为空白组、模型组、格拉司琼组(盐酸格拉司琼灌胃2.70mg/kg)和消痰和胃组(消痰和胃方灌胃2.448g/kg),观察顺铂(6mg/kg)腹腔注射造模后0-72h高岭土与正常饲料摄食量;采用双抗体夹心酶联免疫吸附法(ELISA)法检测72h时大鼠血清、脑干、小肠组织中5-羟色胺(5-HT)及其代谢产物5-羟基吲哚乙酸(5-HIAA)的含量。结果:顺铂干预后0-72h各组正常饲料摄食量存在统计学差异(P<0.01),消痰和胃组与模型组比较可明显增加大鼠正常饲料摄入量,并且优于格拉司琼组(P<0.01);各组血清与脑干5-HT含量存在统计学意义(P=0.0119和P=0.0000),消痰和胃方可降低顺铂诱导后血清与脑干中5-HT含量,并且优于格拉司琼(P<0.05)。结论:消痰和胃方可改善顺铂诱导的厌食症状,其防治化疗延迟性呕吐的机制可能与降低血清与脑干5-HT含量有关。

Objective： To investigate the mechanism and effect of Xiaotan Hewei Decoction in preventing chemotherapy-induced delay emesis.Methods： Wistar rats were injected intraperitoneally with cisplatin 6mg/kg to induce pica.The Xiaotan Hewei Decoction was administered intragastrically（2.448g/kg） for 3d before and after injection respectively with granisetron hydrochloride（2.70mg/kg） as control drug.Kaolin and common feed consumption were recorded in the following 72h.The content of 5-hydroxytryptamine（5-HT） and 5-Hydroxyindoleacetic acid（5-HIAA） in brain stem,small intestine and serum were determined by ELISA.Results： The Xiaotan Hewei Decoction could significantly improve anorexia induced by cisplatin in rats more than granisetron hydrochloride（P0.01）.The Xiaotan Hewei Decoction could reduce the high content of 5-HT in serum and brain stem（P=0.0119 and P=0.0000）.The content of 5-HT in the Xiaotan Hewei Decoction group was lower than that in the granisetron hydrochloride group（P0.05）.Conclusion： The Xiaotan Hewei Decoction can improve chemotherapy-induced anorexia.The mechanism of Xiaotan Hewei Decoction in preventing chemotherapy-induced delay emesis may be on the base of regulating the content of 5-HT in brain stem and serum.