胶体金法与液相色谱-质谱法MDMA检测比较

A comparison study of the detection of MDMA in urine between liquid chromatography mass spectrometry and colloidal gold strip

目的:通过胶体金法对药物滥用患者尿液中3,4-亚甲基二氧基甲基苯丙胺(MDMA)及代谢产物进行检测,并与液相色谱-质谱法检测结果进行比较以验证其检测效果。方法:选取广州市脑科医院物质依赖科收治的药物滥用患者,分别采用胶体金法和液相色谱-质谱法检测其尿液中MDMA及相关成分,计算分析胶体金法检测试剂的灵敏度、特异度、Youden指数与总符合率。结果:在以液相色谱-质谱法为金标准的检测结果对比中,胶体金法检测试剂的灵敏度100.00%,特异度98.88%,Youden指数0.9888,样本检测总符合率98.97%,Kappa=0.936,P=0.00。结论:与液相色谱-质谱法相比较,胶体金法具有操作简便、直观、快速、省时的特点,其特异性、敏感性较高,具有良好的检测效果。

Objective：To verify the determination of colloidal gold strip on detecting 3,4-methylened-ioxymethamphetamine （ MD- MA） and its metabolite in urine of the drug addicts compared with liquid chromatography mass spectrometry（LC/MS）. Methods： Se- lect the drug addicts treated in substance dependence department of Guangzhou brain hospital as the objects in oar study. Detect MD- MA and its metabolite in urine of the drug addicts by way of LC/MS and colloidal gold strip respectively. Calculate and analyze the in- dex of sensitivity, specificity, Youden＇ s index and total coincidence rate based on the detection results of colloidal gold strip when compared with the golden-standard（LC/MS）. Results： Compared with LC/MS, the sensitivity, specificity, Youden＇s index and total coincidence rate of the detection results of colloidal gold strip were 100. 00% , 98.88% , 0. 9888 and 98. 97% respectively. Kappa = 0. 936, P = 0. 00. Conclusion： Compared with LC/MS, colloidal gold strip is more simple and intuitive, faster and more time-saving in operation, and is better sensitivity and specificity in determination.