烟草苯丙氨酸解氨酶活力与多酚含量的关系研究

Study on the Relationship between Activity of Phenylalanine Ammonia Lyase(PAL) and Polyphenol Content in Tobacco Seedling

[目的]测定不同品种烟草幼苗中苯丙氨酸解氨酶(PAL)活力、比活力以及多酚含量,研究PAL活力与多酚含量的关系。[方法]3个烟草品种分别为西1#、东2#和中3#。[结果]不同酶提取缓冲液对测定的PAL活力值有影响,其中10%PVP的效果最好;烟草幼苗中的多酚含量高,则其PAL活力及比活力都较高,而且PAL活力与酶提取液中β-巯基乙醇的浓度呈正向相关关系。在试验材料中,多酚含量没有达到抑制PAL活力的水平。[结论]该研究可为探索改进PAL活力测定方法,明确多酚含量与PAL酶活力间的关系提供参考依据。

[Objective] The purpose was to determine the activity of phenylalanine ammonia lyase（PAL）,specific activity and polyphenol content in different varieties of tobacco seedling,and study the relationship between PAL activity and polyphenol content.[Method] Three tobacco variety was west 1#,east 2# and middle 3#,respectively.[Result] The enzyme extraction buffers had some effects on PAL activity value,in which 10%PVP＇s effects was the best.The PAL activity of the tobacco variety seedling with higher polyphenol content was higher,and PAL activity showed a positive correlation with the concentration of β-mercaptoethanol in enzyme extraction buffer.Polyphenol content could hardly inhabit the activity of PAL.[Conclusion] The study provides a reference basis for exploring modified determination method of PAL activity,and confirm the relationship between PAL activity and polyphenol content.