HPLC法测定眼保Ⅰ号胶囊中葛根素和芍药苷的含量

Determination of Puerarin and Paeoniflorin in Yanbao I Capsule by HPLC

[目的]建立眼保Ⅰ号胶囊中葛根素和芍药苷的含量测定方法。[方法]色谱柱为Diamonsil C18(2)(4.6 mm×250 mm,5μm);流速为1 ml/min;柱温为25℃;葛根素流动相为无水甲醇-水(28∶72,V/V),检测波长为250 nm;芍药苷流动相为乙腈-浓度0.1%磷酸溶液(14∶86,V/V),检测波长为230 nm。[结果]葛根素在0.001 18～0.007 08μg/ml浓度范围内浓度与峰面积的线性关系良好(r=0.999 9),平均回收率102.4%,RSD为1.17%(n=6);芍药苷在0.044～0.261μg/ml浓度范围内浓度与峰面积的线性关系良好(r=0.999 9),平均回收率97.8%,RSD为1.71%(n=6);眼保Ⅰ号胶囊内容物,葛根素和芍药苷的平均含量分别为0.055和0.168 mg/g。[结论]该方法简单,准确,重现性好,可用于眼保Ⅰ号胶囊中葛根素和芍药苷的含量检测。

[Objective] To establish the method for determining puerarin and paeoniflorin in Yanbao I capsule.[Method] The HPLC analysis used chromatography glass column Diamonsil C18（2）（4.6 mm×250 mm,5 μm） with the detection wavelength at 250 nm for puerarin and 230 nm for paeoniflorin and the column temperature was set at 25 ℃.Following the injection of sample solution,an elution was initiated at a flow rate of 1 ml/min with anhydrous methanol-water（V/V,28∶ 72） for Puerarin and acetonitrile-0.1% phosphoric acid（V/V,14∶ 86） as mobile phase.[Result] The linear range of puerarin was from 0.001 18 to 0.007 08 μg/ml（r=0.999 9）.The average recovery of sample was 102.4% with RSD of 1.17%（n=6）.The linear range of paeoniflorin was from 0.044 to 0.261 μg/ml（r=0.999 9）.The average recovery of sample was 97.8% with RSD of 1.71%（n=6）.In Yanbao I capsule,the content of puerarin and paeoniflorin could reach 0.055 and 0.168 mg/g,respectively.[Conclusion] The method is simple,accurate and repeatable,which is suitable for determining puerarin and paeoniflorin in Yanbao I capsule.