HPLC法测定落葵果实中黄酮类化合物的含量

Determination of Flavonoids Content in Basella rubra L.Fruits by HPLC

[目的]建立落葵果实中黄酮类化合物的含量测定方法。[方法]以芦丁为对照品,色谱柱为Eclipse Zorbax XDB-C18(4.6 mm×150 mm,5μm),流动相为无水甲醇-水(55∶45,V/V),流速为0.8 ml/min;检测波长为358 nm,柱温为室温。[结果]在色谱条件下芦丁标准曲线回归方程为Y=20.889X-17.147(R2=0.998 2),在2.00～40.0μg/ml浓度范围内,浓度与峰面积的线性关系良好,平均回收率大于96%,RSD小于2.0%,用该法测得落葵果实黄酮含量为179.0μg/g。[结论]该方法样品处理简单,检测高效快速,结果准确可靠,重现性良好,适合于落葵果实中黄酮类化合物的含量测定。

[Objective] To establish the determination method for flavonoids content in Basella rubra L.fruits.[Method] With rutin as the reference substance,chromatographic column was Eclipse Zorbax XDB-C18（4.6 mm×150 mm,5 μm）;mobile phase was anhydrous methanol-water（V/V,55：45）,flow rate was 0.8 ml/min;detection wavelength was 358 nm;column temperature was room temperature.[Result] Under the chromatographic conditions,regression equation of rutin standard curve was Y = 20.889X-17.147（R2 = 0.998 2）.Linear regression was good between the concentration of 2.00 and 40.0 μg/ml.The average recovery rate was greater than 96% and RSD was smaller than 2.0%.Flavonoids content in B.rubra fruits.was 179.0 μg/g by this method.[Conclusion] This method was simple,rapid,efficient,repeatable,accurate and reliable,and was suitable for the content determination of flavonoids in B.rubra fruits.