水法提取普洱茶茶多糖条件优化

Optimization of Water Extraction for Tea Polysaccharide from Pu'er Tea

[目的]筛选热水浸提法提取普洱茶中水溶性茶多糖的最佳条件。[方法]采用热水浸提法提取普洱茶中的水溶性茶多糖,根据浸提时间、浸提温度、料液比、提取次数对多糖得率的影响,通过正交试验得到普洱茶水溶性多糖浸提工艺优选因素组合。[结果]影响普洱茶茶多糖得率的因素主次顺序为浸提时间>提取次数>浸提温度>料液比;最佳浸提条件为固液比1∶20 g/ml、温度90℃、时间1.5 h、浸提2次;在最佳浸提工艺条件下进行浸提试验,得到的最佳提取率是54.5%。[结论]为普洱茶中茶多糖的提取提供了依据。

[Objective] The aim was to screen the optimum conditions for extracting water-soluble tea polysaccharide from Pu＇er tea by hot water extraction method.[Method] By using hot water extraction method to extract water-soluble tea polysaccharide,according to the effects of extraction time,temperature,solid-liquid ratio,times on polysaccharide yield,the optimum factors were obtained through orthogonal test.[Result] The order of influencing factors for tea polysaccharide extraction from Pu＇er tea are extraction time extraction times extraction temperature solid-liquid ratio;the optimum extraction conditions are solid-liquid ratio 1∶20 g/ml,temperature 90 ℃,time 1.5 h,extraction 2 times;under the above conditions,the optimum extraction rate is 54.5%.[Conclusion] The study will provide basis for tea polysaccharide extraction from Pu＇er tea.