木质纤维素产乙醇分步及同步糖化发酵工艺研究

Process Research on Separate Hydrolysis and Fermentation(SHF) and Simultaneous Saccharification and Fermentation(SSF) of Ethanol Production by Lignocellulose

[目的]探究分步糖化发酵和同步糖化发酵工艺在最佳条件下产乙醇的效率。[方法]结合水解、发酵两阶段的最佳条件,设计了分步糖化发酵和同步糖化发酵过程;分步糖化发酵分别采用了水解和发酵过程的最佳条件,同步糖化发酵分别采用了两阶段的最佳温度。[结果]分步糖化发酵过程中,上清液发酵和混合液发酵产乙醇效果无明显差别,其中混合液发酵能更好地发挥酵母菌的活力;同步糖化发酵过程中,35℃条件下乙醇产量更高,其木糖产量高于纯水解过程中的木糖产量。[结论]在试验条件下,同步糖化发酵产乙醇效率高于分步糖化发酵。

[Objective] The research aimed to explore the efficiency of ethanol production under the optimal conditions of separate hydrolysis and fermentation（SHF） and simultaneous saccharification and fermentation（SSF）.[Method] Under the optimal conditions of hydrolysis and fermentation,SHF and SSF process were designed respectively.SHF process was conducted under the optimal conditions of hydrolysis and fermentation separately,while SSF process was carried out at the optimum temperature of hydrolysis and fermentation.[Result] In SHF process,the efficiency of ethanol production was similar in both supernatant fermentation and Mixture fermentation,and yeast was more active in the mixture fermentation.In SSF process,the efficiency of ethanol production was higher at 35 ℃,and the production of xylose was higher than that in hydrolysis process.[Conclusion] Under the experimental conditions,the efficiency of ethanol production in simultaneous saccharification and fermentation was higher than that in separate hydrolysis and fermentation.