正交试验优选菊花口服液的提取工艺

Optimization of the Preparation Technology of Chrysanthemum Oral Solution by Orthogonal Design

[目的]探索菊花口服液的制备工艺。[方法]以乙醇浓度、料液比、回流时间和回流次数作为因素,以总黄酮的含量为指标,并采用紫外分光光度法进行测定,采用L9(34)正交试验优化菊花口服液的提取工艺;将提取液过滤、浓缩,加水稀释,静置,加滑石粉助滤,加辅料配制,制备口服液。[结果]最佳提取工艺为:乙醇浓度60%,料液比1∶20(g/ml),回流时间1 h,回流次数1次;在此条件下,总黄酮含量最高为3.108 6%;总黄酮的标准曲线为Y=25.455X+0.004 7,R2=0.999 8;制得的口服液外观呈棕黄色的澄清液体,久置可有少量轻摇易散的沉淀;味甜。[结论]该工艺简便,制成成品质量稳定。

[Objective] To discuss the preparation technology of Chrysanthemum oral solution.[Method] Taking the content of total flavonoids as index,the extraction technology of Chrysanthemum was optimized by means of L9（34） orthogonal experiment with ethanol concentration,solid-liquid ratio,reflux time,and reflux times as investigated factors.The content of total flavonoids was determined by UV spectrophotometry.The Chrysanthemum extract was filtered,concentrated,diluted with water,filtered with the aid of talcum powder,and finally blended with other auxiliary materials to prepare Chrysanthemum oral solution.[Results] The optimal extraction technology was： ethanol concentration of 60%,solid-liquid ratio of 1∶20 g/ml,reflux time of 1 h,and reflux times of 1.Under this condition,the content of total flaovnoids could reach 3.1086%.The regression equation of total flavonoids was Y=25.455X＋0.004 7,R2=0.999 8.The prepared oral solution was a kind of brown,transparent and sweet liquid,in which there would be little sediment over a long time.[Conclusion] This is a simple technology,by which stable finished products are prepared.