摘要
将60 Co γ射线用于水稻花药培养育种中 ,结果表明 ,5~ 1 0Gy的低剂量处理接种前花药能提高花培绿苗诱导率 ,1 5 0~ 2 0 0Gy的高剂量急性处理水稻F0 代干种子 ,接种M1代花药 ,对花药绿苗诱导效果不明显 ,但能提高花培后代植株的变异频率 ;接种 2 5 0Gy剂量处理F0 代种子的当代材料花药 ,会降低花药培养力。
The results of applying 60 Co γ ray to rice anther culture showed that the frequencies of the calli induction and the green plantlet differentiation increased when anthers was treated with 5 10 Gy of gamma ray before inoculation.The cultured ability could not increase but the variation frequencies of anther green plants increased.The anther culture ability of MF 1 anthers from F 0 seeds irradiated with 150 200Gy of gamma ray would lower down.It was not very beneficial to induction of calli when the dose of irradiation was over 250Gy.
出处
《西南农业学报》
CSCD
北大核心
2000年第1期17-20,共4页
Southwest China Journal of Agricultural Sciences