洋参御糖丸对STZ-DM模型大鼠肝脏结构影响的实验研究

Effect of YTW on the Liver Organization Structure in 2DM Animal Models Induced by STZ and High Fat Diet

复制2DM-STZ模型大鼠,观察洋参御糖丸对2DM大鼠肝脏形态学的影响,为临床用药提供依据。方法:100只Wistar大鼠,12只为空白对照组(K)给予普通饮食,其余88只给予腹腔注射STZ联合高脂饮食喂养四周。成功造模75只,分为5组:模型组(M)、西药对照组(Xd)、中药对照组(Zd)、御糖丸低剂量组(Yd)、御糖丸高剂量组(YG)。实验结束,观察各组大鼠肝脏进行光镜、电镜形态学观察。结果:与正常对照组比较,各治疗组上有不同程度的改善肝细胞结构的影响,其中御糖丸高剂量组有较显著的治疗作用。结论:1.本实验用高脂饮食联合链脲佐菌素(STZ)法成功复制2DM模型;2.各治疗组对大鼠肝脏起到不同程度的结构影响,其中洋参御糖丸起到改善肝细胞结构的治疗作用。

Objective: To investigate the effect of YTW on the liver structure changes of 2DM rats in model group and different treatment groups.Methods: Wistar male rats were 100,including 12 in the control group which were given free water intake.The model group(including 88 rats) with high sugar and high fat diet were given the injection of STZ in abdominal cavity for four weeks later.At last we selected the successful modeling and blood glucose levels close to 75 rats.Then they were divided into five groups stochastically(each group of 15 rats): model group(M),YU Di group(YD),YU Gao group(YG),Zhong Dui group(Zd-Liu Wei Di Huang Wan),Xi Dui group(Xd-metformin).At the end of the experiment,pathological section of all rats′ liver were made and stained by light microscope and electric mirror for observation.Result: Compared with normal control group(K),to some extent,liver structure has different changes after treatment,especially excellence in high dose of YG group.That could be seen in two treatment groups(YD,YG),in which rats' liver structure had been affected.Conclusion: Copied STZ in diabetic rats and high fat diet,and different liver damages of rats have been seen in groups.It can be concluded that YTW can alleviate the 2DM rats′ liver structure damages obviously after treatment,that is,YTW has a certain therapeutic function on diabetic animal models.