心脑通络液对脑梗死大鼠脑组织Glu、Asp含量的影响

Effect of XinTaoTongLuoYe on Contents of Brain Tissues Glu and Asp in Rats With Cerebral Ischemia

目的:观测心脑通络液对脑梗死大鼠脑组织Glu、Asp含量的影响。方法:将健康雄性SD大鼠随机分为脑心通对照组(对照组)、心脑通络液低剂量组(低剂量组)、心脑通络液高剂量组(高剂量组)、假手术组和模型组共5组。采用线栓法制备大脑中动脉局灶性脑缺血模型,各治疗组从大鼠术前5天至处死前,每日给药,每日1次。分别在造模后6h、12h、24h、48h 4个时间点进行指标检测。采用高效液相色谱仪测定Asp、Glu的含量。结果:Glu含量测定结果显示,脑梗塞6h,模型组脑组织Glu含量开始显著增加,12h有所下降,但仍有显著性差异(P<0.01),24h、48h无统计学意义(P>0.05);各治疗组与同点模型组比较,在脑梗塞6h、12h后Glu含量均有不同程度降低,且均有显著性差异(P﹤0.01或P﹤0.05),以高剂量组降低最为显著,24h、48h各治疗组Glu含量有所增加,但除48h的对照组、高剂量组外,均无统计学意义(P>0.05);高、低剂量组与同点对照组比较,各高剂量组(除48h)有统计学意义(P﹤0.01或P﹤0.05);Asp含量检测结果为:模型组脑组织在脑梗死6h时Asp含量开始增加,到48h时Asp含量达到顶峰(P<0.01);各治疗组与模型组相比,大鼠脑组织中的Asp的含量均有降低(P﹤0.01),尤其以高剂量组表现最为明显;高、低剂量组(除48h高剂量组外)与同时间点对照组比较,无显著性差异(P>0.05)。结论:心脑通络液能够有效地降低急性期脑梗死大鼠脑组织中Glu、Asp的含量,从而抑制兴奋性氨基酸的毒性作用,对脑梗塞的恢复起到积极有效的治疗作用。

To observe the effect of XinNaoTongLuoYe on the content of Glu and Asp in brain of focal cerebral ischemia rats. Methods： The Healthy ats in SD with the weight of 250 -300gs were divided into five groups at random as examination of infarct volume, that is, Buchangnaoxintong control groups （called control group）, low - dose of XinNaoTongLuoYe group （ called low - dose group ） , high - dose of XinNaoTongLuoYe group （ called high - dose group）, sham operation group and model group. According to the report, by inserting nylon thread on the middle cerebral arteries to make the model of cerebral infarction, rats in each treatment group were given medicine through watering stomach every day during preoperative five days before death. After making the model rats successfully in 6h, 12h,24h,48h, the changes of the content of Glu and Asp in brain were examined by high performance liquid chromatography （HPLC）. Results： The monitoring results of Glu content in brain showed that model group was increased in 6h after cerebral ischemia, though it decreased after12h, there was still a significant difference（ P 〈0.01 ） ;Compared with the model group at the same time, Glu content of each of treatment groups differently reduced in 6h,12h after cerebral ischemia, and the difference was obviously significant（ P 〈 0. 01 or P 〈 0.05 ）, especially the high -dose group. However, in 24h ,48h（ except the high -dose and low -dose groups in 48h）the Glu content increased slightly, and there was no statis- tical significance about the change（ P 〉 0. 05 ） ;Each of high and low -dose groups compared with the control group at the same time, the alteration of Glu content of each high - dose group （ except 48h） had a statistical significance （ P 〈 0. 01 or P 〈 0. 05 ）. About the monitoring results of content of Asp in brain , the data of model group in brain began to increase in 6h after cerebral ischemia, and it reached the peak in 48h（ P 〈 0. 01 ） ; Compared with the model group at the same time, the Asp content of brains of each of treatment groups presen- ted a significant difference（ P 〈 0.01 or P 〈 0. 05 ）, especially the high- dose group; Each of high and low dose groups compared with the control groups at the same time, the alteration of Asp content of each group （ except 48h high - dose groups） had no statistical significance （ P 〉 0.05 ）. Conclusion ： The toxic effects of excita- tory amino acids can be reduced by neurons protection of XinNaoTongLuoYe, which can effectively bring down the content of Glu （12h, 24h） and Asp in brain,and it is beneficial for cerebral infarction recovery.