杨树体细胞融合研究

A Study on Poplar Somatic Hybridization

为克服有性杂交障碍 ,利用原生质体电融合技术开展杨树体细胞融合研究。本研究选择杨树不同种或无性系分离原生质体 ,并应用IOA和X 射线分别对融合双亲处理 ,使其失去分裂能力 ,筛选杂种细胞 ,并探索杨树原生质体融合的最适电场参数与培养条件 ,建立杨树体细胞电融合体系。首次得到了美洲黑杨 +胡杨、青杨 +胡杨、美洲黑杨 +青杨等体细胞杂种愈伤组织。其中美洲黑杨 +胡杨、青杨 +胡杨体细胞杂种愈伤组织可在附加 0 .4%氯化钠的培养基上生长 。

Somatic protoplast fusion has turned out to be a suitable method to overcome barriers of sexual hybridization. The actual method for successful somatic hybridization between different species of poplar such as Populus euphratica Oliv., P.cathayana Rold., P.deltoides × P.simonii NL 80106, P.euramericana cv. I 69 and P. tomentosa and different isolation materials were selected and treated with IOA and X ray irradiation to suppress division of the unfused protoplasts. Preliminary studies were performed to determine the dose of X ray irradiation to be used during fusion experiments. The optimal condition applied for fusion of the cells was also studied. One week after protoplast fusion the first division could be observed and after one month the calli 1mm long were formed. Mass of the calli derived from the fused cells between Populus deltoides × P. simonii NL 80106 and P. euphratica Oliv., P.cathayana Rold. and P.euphratica Oliv. , P.deltoides × P.simonii NL 80106 and P.cathayana Rold. were obtained, the former two of which could grow on the medium added with 0.4% NaCl. It indicated that the calli had salt resistant character of P.euphratica Oliv. which is one of the fusion partners.