精脒和腐胺对人胎儿脾LAK活性的影响

Synergy of spermidine or putrescine and recombinant IL-2 in the generation of human fetal spleen LAK cell cytotocixity

本研究表明,精脒、腐胺本身均不能使人胎脾单个核细胞(FSMC)转化为LAK细胞,但与5u/ml重组白细胞介素2(rIL-2)伍用,可以增强人胎脾LAK细胞活性;精脒,腐胺不能直接刺激细胞增殖,但能协同亚适剂量Con A诱导淋巴细胞增殖;精脒或腐胺本身可刺激FSMC的IL-2受体(IL-2R)的表达,并能协同rIL-2进一步提高人胎脾LAK细胞IL-2R的表达;精脒、腐胺均可使FSMC及胎脾LAK细胞内鸟氨酸脱羧酶(ODC)活性增强,而以腐胺的作用尤为显著。

The effect of spermidine and putrescine on human fetal spleen LAK cell cytotoxicity was evaluated. The results showed that (a) although spermidine or putrescine was incapable of inducing LAK function. addition of which at initiation of fetal spleen mononuclear cells cultures stimulated with a lower dose of rIL-2 could augmented the induction of cytotoxicity and the synergistic effect had been observed in a wide range of spermidine or putrescine concentration; (b) in addition of the two materials. Con A-induced lymphocyte proliferation could be increased; (c) IL-2 receptor expression on human fetal spleen mononuclear cells and LAK cells could be enhanced slightly by spermidine or putrescine; (d) the level of ornithine decarboxylase in fetal spleen mononuclear cells and LAK cells could be raised by spermidine or putrescine. The study afforded us valuable information about immune function of polyamines and the clinic use of fetal spleens.