巨噬细胞细胞毒因子分泌调节的研究

Regulation of TNF-like cytotoxic facor(s) production by murine peritoneal macrophage

小鼠腹腔巨噬细胞(Mφ)产生细胞毒因子(Mφ—CF),需激活和刺激两个信号。激活信号(A23187)单独不能诱导Mφ—CF,但能促进刺激信号(LPS)的诱导作用。活化的Mφ经LPS作用2小时内开始分泌Mφ—CF,8小时达(?),后逐降。对酵母多糖的吞噬伴有一定程度的Mφ—CF分泌,但吞噬乳胶颗粒则否。数株肿瘤细胞有明显的刺激Mφ—CF分泌的作用。PKc激活剂不能刺激Mφ—CF的产生。

Effects of the activiating and stimulating signals on the production of TNF-like cytotoxic factor (s) [Macrophage-derived cytotoxic factor(s), Mφ-CF] were investigated. Although activiating signals (Calcium ionophore A23187 and Macrophage-activiating factor, MAF) did not induce the production of Mφ-CF by themselves, they were significantly synergistic in the production of Mφ-CF induced by stimulators. Upon exposing to Iipopolysaccharide (LPS) Mφ-CF appeared in culture supernatant within 2 hrs, reached a peak after about 8 hrs and then declined gradually. In addition to LPS, opsonized zymasan A and several tumor cell lines also induced Mφ-CF production. whereas latex beads and normal allogenic spleen lymphocytes had no effect on the production. Finally the stimulating role of LPS, zymosan A and tumor ceils could not be mimicked by protein kinase C (PKc) activiators (phorbol myristate acetate, PMA and calcium ionophore A23187). These results suggested that the induction of Mφ-CF production be stimulus-specific and PKc-independent.