应用单抗分析结晶天花粉蛋白的抗原决定簇

Mapping of antigenic determinants of THF protein with monocional antibodies

本文报道用制备的22株单克隆抗体为探针,采用ELISA双抗体相加试验以分析结晶天花粉蛋白上抗原决定簇的结果。经集群分析法处理,可将22株单抗集群为不同的六组。由此提示,结晶天花粉蛋白上有六个不同的、可被单抗识别的抗原决定簇。此数目与我们对结晶天花粉蛋白上B细胞识别的抗原决定簇进行计算预测所得到的结果相吻合。这对于从分子水平进一步探讨结晶天花粉蛋白结构与功能之间的关系奠定了基础。

Using 22 monoclonal antibodies (McAbs) as probe to analyse the antigenic determinants of crystallized Tian Hua Fen (THF) or Trichosanthin protein in ELISA additivity test was reported. The 22 McAbs were come from five fusions, each from different fusion well. The isotype of 22 McAbs were identified by immunodiffusioo test, 20 McAbs belong to IgG_1 subclass, 1 IgG_(2a) subclass and 1 IgM class. Based on the results obtained from ELISA additivity test, 22 McAbs could be clustered into six distinct groups. It indicates that there are six different antigenic determinants in crystallized THF, each can be recognized by some McAbs. The results coincide with the calculation of B cell recognized antigenic determinants obtained by prediction from amino acid sequences of THF protein, The work has laid a foundation for further exploring the structure-function relationship of THF protein on molecular level .