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RAB-27 and its effector RBF-1 regulate the tethering and docking steps of DCV exocytosis in C.elegans 被引量:1

RAB-27 and its effector RBF-1 regulate the tethering and docking steps of DCV exocytosis in C.elegans
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摘要 The molecular mechanisms by which dense core vesicles(DCVs) translocate,tether,dock and prime are poorly understood.In this study,Caenorhabditis elegans was used as a model organism to study the function of Rab proteins and their effectors in DCV exocytosis.RAB-27/AEX-6,but not RAB-3,was found to be required for peptide release from neurons.By analyzing the movement of DCVs approaching the plasma membrane using total internal reflection fluorescence microscopy,we demonstrated that RAB-27/AEX-6 is involved in the tethering of DCVs and that its effector rabphilin/RBF-1 is required for the initial tethering and subsequent stabilization by docking. The molecular mechanisms by which dense core vesicles (DCVs) translocate, tether, dock and prime are poorly understood. In this study, Caenorhabditis elegans was used as a model organism to study the function of Rab proteins and their effectors in DCV exocytosis..RAB-27/AEX-6, but not RAB-3, was found to be required for peptide release from neurons. By analyzing the movement of DCVs approaching the plasma membrane using total internal reflection fluorescence microscopy, we demonstrated that RAB-27/AEX-6 is involved in the tethering of DCVs and that its effector rabphilin/RBF-1 is required for the initial tethering and subsequent stabilization by docking.
出处 《Science China(Life Sciences)》 SCIE CAS 2012年第3期228-235,共8页 中国科学(生命科学英文版)
基金 supported by the National Basic Research Program of China(Grant No. 2010CB833701) the National Natural Science Foundation of China(Grant Nos. 30870564 and 90913022) the CAS Project(Grant No.KSCX2-SW-224)
关键词 EXOCYTOSIS dense core vesicles RAB-3 RAB-27 C. elegans total internal reflection fluorescence microscopy 直流电压 线虫 对接 RBF 胞吐 牵引 荧光显微镜 分子机制
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