马铃薯对甘露醇诱导静脉炎模型兔血清IL-6水平及局部VEGF表达的影响

Effect of potato external application on serum IL-6 level and VEGF expression in local tissue of rabbit with phlebitis induced by mannitol

目的:探讨马铃薯贴敷对甘露醇所致静脉炎模型兔血清IL-6水平和局部组织中VEGF表达的影响。方法:将25只日本大耳白兔随机分为5组:生理盐水组、模型组、硫酸镁组、马铃薯片贴敷组和马铃薯泥贴敷组,每组5只。后4组静脉给予体积分数20%甘露醇建静脉炎模型。末次贴敷后,取静脉穿刺点周围耳廓组织,采用免疫组化SABC法检测VEGF的表达;采血,采用双抗体夹心ABC-ELISA法检测血清IL-6水平。结果:5组VEGF和IL-6水平差异均有统计学意义(F=37.307,50.662,P<0.001)。模型组两指标水平明显高于生理盐水组,硫酸镁组、马铃薯片贴敷组和马铃薯泥贴敷组两指标水平较模型组降低(P<0.05),其中马铃薯泥贴敷组效果更明显,但这3组两指标水平仍高于生理盐水组(P<0.05)。结论:马铃薯贴敷可有效防治甘露醇所致的静脉炎;马铃薯泥贴敷效果优于马铃薯片。

Aim：To observe the effect of potato external applying on phlebitis caused by mannitol in rabbits.Methods：A total of 25 healthy large-eared Japanese rabbits were randomly divided into five groups：negative group,model group,magnesium sulfate group,potato slice and potato paste group.The expression of VEGF in local tissue and serum IL-6 were detected by immunohistochemical SABC method and ABC-ELISA,respectively.Results：There were significant differences in serum level of IL-6 and the expression of VEGF among the 5 groups（F=50.662,37.307,P0.001）.They were higher in model group than in negative group（P0.05）,and they were lower in the 3 other groups than in model group,especially in potato paste group（P0.05）,but still higher than those in negative group（P0.05）.Conclusion：Potatoes compress is effective in prevention and treatment of phlebitis induced by mannitol,and the effect of potato paste is superior to potato slice.