八核铜络合物对SGC-7901细胞生长的影响

Effect of eight nuclear copper complexes on growth of SGC-7901 cell

目的:研究八核铜络合物(N-Cu)对人胃癌SGC-7901细胞生长的影响,探讨N-Cu抗肿瘤活性的相关机制。方法:体外常规培养人SGC-7901细胞,用不同质量浓度的N-Cu(3.125、6.250、12.500、25.000、50.000、100.000mg/L)分别处理24、48和72h后,MTT法检测SGC-7901细胞增殖抑制率,计算IC50。分别用12.5、25.0和50.0mg/LN-Cu处理SGC-7901细胞,倒置显微镜和扫描电镜下观察细胞形态的改变,流式细胞仪测定SGC-7901细胞周期和凋亡,Western-blot法检测Caspase-3蛋白的表达。结果:N-Cu作用24、48和72h对SGC-7901细胞的IC50分别为73.45、33.71和22.78mg/L。随着N-Cu质量浓度的增加和作用时间的延长,扫描电镜下可见细胞微绒毛逐渐消失,胞质收缩,细胞呈球形,早期凋亡细胞形成膜包裹的凋亡小体凸出于细胞表面。N-Cu可剂量依赖性和时间依赖性地抑制SGC-7901细胞的增殖,将其阻滞在G0/G1期(F组间=3586.750,F时间=418.133,F交互=224.383,P均<0.001)。N-Cu可剂量依赖性地促使SGC-7901细胞发生凋亡(F=66.932,P<0.001),增强Caspase-3蛋白的表达(F=55.133,P<0.001)。结论:N-Cu可通过诱导Caspase-3蛋白的表达,参与SGC-7901凋亡的调节,抑制细胞增殖。

Aim：To explore the effect of eight nuclear copper complexes（N-Cu） on the growth of SGC-7901 cell.Methods：SGC-7901 cells were treated with different concentrations（3.125,6.250,12.500,25.000,50.000,and 100.000 mg/L） of N-Cu for 24,48,and 72 h,the cell proliferation was detected by MTT assay,and IC50 was calculated.SGC-7901 cells were treated with different concentrations（12.5 mg/L,25.0 mg/L,50.0 mg/L） of N-Cu for 24,48,and 72 h,cell cycle and apoptosis rate were detected using flow cytometry,and the expression of Caspase-3 protein was detected using Western-blot assay.Results：IC50 of N-Cu to SGC-7901 cells was 73.45,33.71 and 22.78 mg/L at 24,48 and 72 h.In time-and concentration-dependent manner,N-Cu could inhibit SGC-7901 proliferation,arrested it at G0/G1 phase（Fconcentration=3 586.750,Ftime= 418.133,P0.001）,increased the expression of Caspase-3 protein（F=55.133,P0.001）,and promoted apoptosis（F=66.932,P0.001）.Conclusion：N-Cu could restrain the cell proliferation by regulating the apoptosis of SGC-7901 cells through inducing the expression of Caspase-3 partly.