非黑色素性皮肤癌组织中血管内皮生长因子及其受体1和受体2的表达

Expressions of VEGF,Flt-1 and KDR protein in skin SCC and BCC tissue

目的:检测皮肤鳞状细胞癌(SCC)和基底细胞癌(BCC)组织中血管内皮生长因子(VEGF)及其受体1(Flt-1)、受体2(KDR)的表达。方法:应用免疫组化SP法检测26例皮肤SCC、18例BCC组织中VEGF、Flt-1和KDR的表达。取10例正常皮肤组织标本作对照。结果:皮肤SCC、BCC和正常皮肤组织中VEGF的表达水平分别为(129.05±9.93)、(119.40±9.37)及(85.25±12.98),Flt-1的表达水平分别为(130.48±12.02)、(116.90±13.45)及(82.19±9.15),KDR的表达水平分别为(136.60±6.67)、(120.95±6.33)和(85.43±9.33),3组间VEGF、Flt-1和KDR蛋白的表达差异均有统计学意义(F分别为83.418、78.137和169.125,P均<0.001)。皮肤SCC组织中VEGF与Flt-1(r=0.773,P<0.001)、VEGF与KDR(r=0.676,P<0.001)、Flt-1与KDR(r=0.718,P<0.001)的表达均呈正相关。结论:VEGF及其受体的过表达可能与非黑色素性皮肤癌的发生有关。

Aim：To detect the expressions of vascular endothelial growth factor（VEGF）,VEGF receptor-1（Flt-1） and receptor-2（KDR） in cutaneous squamous cell carcinoma（SCC）and basal cell carcinoma（BCC） tissue.Methods：The expressions of VEGF,Flt-1 and KDR in 26 cases of SCC,18 cases of BCC and 10 normal skin tissue were examined using immunohistochemical SP methods.Results：In SCC,BCC and normal skin tissue,the expression level of VEGF was（129.05±9.93）,（119.40±9.37） and（85.25±12.98）;the level of Flt-1 was（130.48±12.02）,（116.90±13.45） and（82.19±9.15）;the level of KDR was（136.60±6.67）,（120.95±6.33） and（85.43±9.33）.There were significant differences in the expression levels of VEGF,Flt-1 and KDR among the three groups（F=83.418,78.137,and 169.125,P0.001）.In SCC group,the expression levels of VEGF was positively related to those of Flt-1（r=0.773,P0.001）and KDR（r=0.676,P0.001）,and that of Flt-1 was positively related to that of KDR（r=0.718,P0.001）.Conclusion：The over expressions of VEGF,Flt-1 and KDR may play key roles in the development of cutaneous SCC and BCC.