上海人群中HLA-DR7单倍型同时表达两种纯合分型细胞检出的特异性Dw7c及Dw3

Co-expression of two HTC-defined HLA-D specific Dw 3 and Dw 7 c in DR 7 positive halotype among the Shanghai Chinese

采用国际组织相容性会议提供的纯合分型细胞(HTC)和血清对上海地区56例无亲缘关系个体作HLA-A、B、C、D、DR、DQ分型并研究中国人DR-Dw关系后,发现11例Dw3阳性个体中仅5例表达命名相当的DR3特异性,另外6例Dw3阳性者却与DR7及Dw7c(Dw7+Dw17)共同表达于一条单倍型,使同一个体呈现HLA-D“三联体”这样一种未曾报导过的格局。中国人Dw3因而分成两类:一类见于传统的单倍型HLA-DR3-Dw3;另一类组成新单倍型HLA-DR7-Dw7c-Dw30。间接证据表明,后者的出现可能是中国人中一个新的HLA-DQw2分裂体同时参与Dw7c及Dw3功能表达并被HTC所识别的结果。

When 56 unrelated subjects in Shanghai were typed for HLA-A, B, C, D, DR and DQ with the homozygous typing cells (HTCs) and sera of 3 rd Asia-Oceania Histocompatib(?)l(?)tv Workshop Conference, only five of the eleven Dw 3 positive subjects were identified to be associated with the correspondingly designated DR 3. For the other six subjects, Dw 3 were co-expressed with Dw 7 c (Dw7+Dw17) and DR7 in the same halotype, resulting in a Dw 'triplet' when the third D specificity was detected in the other halotypes. The Dw 3 was thus divided into two forms characterized by the two different haplotypes in which it was involved: HLA-B17-DR 3-DRw52-Dw3 and HLA-B13-DR 7-DR w53-DQ w2-Dw7 c-Dw3. With circumstancial evidences, the co-expression of Dw 3 and Dw 7 c was attributable to a tentative DQw 2 split in Chinese, which, as a part of the antigen epitopes, might take part In the expression of Dw3 and Dw7 c specificities and could be recognized by the HTCs.