可溶性IL-2R的纯化及特性研究

Purification and characterization of soluble interleukin 2 receptor

利用溴化氰活化Sepharose 4B制备了免疫吸附亲和层析柱5G_1-Sepharose 4B,从500ml PHA刺激的PBL培养上清中纯化了sIL-2R约160μg。SDS-PAGE显示单一带型,分子量约44kd。纯化的sIL-2R可封闭抗IL-2Rα单克隆抗体与IL-2Rα^+细胞的结合;高浓度时可抑制IL-2依赖的细胞增殖,低浓度时则表现为促进效应。

By using 5 G 1-sepharose 4 B immune affinity column, we recovered approximately 160 μg purified and soluble IL-2 receptor (sIL-2 R ) from 500ml PHA-stimulated human PBL supernatant. Only one band was showed in SDS-PAGE, its molecular weight was about 44 Kd. The purified sIL-2 R, which blocked the binding of anti-IL-2 R monoclonal antibody with IL- 2 R^+ cell, inhibited (at the higher doses) but enhanced (at the lower doses) the growth of IL-2 dependent cells.