摘要
建立快速、简便反相离子对高效液相色谱—电化学检测分析法,一次同时测定生物样品中单胺类递质(NE.E.DA、5—HT)及其前体氨基酸(Tyr、Trp)和主要代谢产物(HVA、5-HIAA共8种化合物。应用该法检测了SRBC免疫小鼠大脑皮质、间脑、脑干8种单胺类化合物的变化规律。免疫后第3天,皮质DA/HVA、间脑NE含量显著降低(P<0.05);第5天,间脑5-HT和脑干5-HIAA含量明显升高(P<0.05)。支持免疫应答可以影响中枢单胺类递质代谢的推论。
A rapid and simple chromatographic procedure using HPLC-ECD is described for simultaneousdetermination of NE. E. DA. 5-HT with their precursor amino acid (Tyr. Trp) and their main metabolites (HVA. 5-HIAA). Using this assay,eight substrates are measured in cortex, diencephalon and brain stem of mice duing immunological response challenged by SRB6 3 days after challenged, the DA HVA in cortex, NE in diencephalon decrease obviously (p<0.05), and 5 days after challenged, 5-HT in diencephalon and 5-HIAA in brain stem increase significantly (p<0.05), which supports the hypothesis that immunological response may act on metabolism of central monoamine transmitters.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
1990年第6期370-373,382,共5页
Chinese Journal of Immunology
关键词
单胺类递质
HPLC
ECD
免疫应答
Reverse phase ion-pair HPLC
Electrochemical detection
Monoamine transmitters
Immunological response