TROP-2基因小干扰RNA转染对前列腺癌细胞黏附、迁移和侵袭的影响

Effects of TROP-2 down-regulation by RNA interence on invasion of human prostate cancer cell

目的观察肿瘤相关钙信号转导蛋白-2(TROP-2)基因小干扰RNA(siRNA)转染对前列腺癌细胞黏附、迁移和侵袭的影响。方法以TROP-2基因siRNA转染人前列腺癌PC-3细胞后,分别用实时荧光定量PCR和Western blot法检测细胞TROP-2 mRNA和蛋白,用MTT法检测PC-3细胞吸光度值(以此判断细胞黏附力),以Tr-answell小室实验观察细胞的迁移、侵袭情况。结果与空载对照组相比,TROP-2 siRNA组TROP-2 mRNA的表达明显下降(P均<0.01)并呈浓度和时间依赖性(P均<0.01);与空白对照组及空载对照组相比,TROP-2 siRNA组TROP-2蛋白的表达明显下降(P均<0.01)并呈浓度和时间依赖性(P均<0.01);与空白对照组相比,TROP-2 siR-NA组细胞黏附力下降(P<0.01)并呈浓度、时间依赖性(P均<0.05);与空白对照组及空载对照组相比,TROP-2siRNA组细胞迁移、侵袭数明显减少(P均<0.01)并呈浓度依赖性(P均<0.01)。结论 TROP-2基因siRNA转染可抑制前列腺癌PC-3细胞黏附、迁移和侵袭。

Objective To explore the effects of TROP-2 gene small interfering RNA（siRNA）on adhesion,migration,and invasion of human prostate cancer cell.Methods After human prostate cancer PC-3 cell was transfected with different dose of TROP-2 siRNA,the expression of TROP-2 mRNA and protein were were determined by real-time quantitative PCR and Western blot assay,respectively.Cell adhesion was evaluated by MTT assay.The migration and invasion was exmined by Transwell,respectively.Results The results from Real-Time quantitative PCR and Western blot showed that TROP-2 mRNA and protein reduced in time-and dose-dependent manners（all P0.01）.The adhesive,migrative and invasive ability of PC-3 cell treated with TROP-2 siRNA decreased compared with control groups（all P0.01）.Conclusion TROP-2 gene may play a pivotal role in adhesion,migration,and invasion of human prostate cancer cell.siRNA targeted TROP-2 can inhibit adhesion,migration,and invasion of human prostate cancer cell.