期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

UC-MSCs对UUO大鼠肾间质ET-1、VEGF、肾小管周Ⅷ因子表达水平的影响 被引量：4

下载PDF
导出
摘要 目的观察人脐血间充质干细胞(UC-MSCs)对单侧输尿管梗阻(UUO)大鼠肾间质中内皮素-1(ET-1)、血管内皮生长因子(VEGF)、肾小管周Ⅷ因子表达的影响。方法 24只大鼠随机分为3组,各8只。模型组与治疗组均行右侧输尿管结扎术,假手术组手术入路方式同模型组,进腹腔后分离右输尿管但不结扎,术后注射生理盐水0.5 mL;模型组术后注射生理盐水0.5 mL;治疗组术后注射UC-MSCs 1×106个(0.5 mL)。术后14 d处死各组动物。术后14 d取梗阻侧肾组织,观察肾小管间质损伤程度,免疫组化法检测肾组织中ET-1、VEGF及肾小管周Ⅷ因子的水平。结果假手术组肾间质中ET-1、VEGF及肾小管周Ⅷ因子相对表达量分别为1.178 8±0.884 2、0.456 3±0.079 3、13.16±8.01,模型组分别为9.623 8±3.323 4、1.346 3±0.612 9、7.36±1.07,治疗组分别为5.243 7±2.657 4、4.290 0±0.594 6、10.29±0.06,三组相比,P均<0.05。肾小管周Ⅷ因子与ET-1表达呈负相关(r=-0.881,P<0.01),与VEGF表达呈正相关(r=0.861,P<0.01)。结论 UC-MSCs可以抑制ET-1的表达,上调VEGF水平,保护肾间质微血管,延缓肾间质纤维化,从而发挥肾保护作用。
作者 孙洞箫 周盾 邱振宇
机构地区 辽宁医学院附属第一医院
出处 《山东医药》 CAS 2012年第10期28-29,共2页 Shandong Medical Journal
基金 辽宁省科学技术计划项目(L2010319)
关键词 肾间质纤维化 人脐血间充质干细胞 Ⅷ因子 血管内皮生长因子 内皮素-1
分类号 R693 [医药卫生—泌尿科学]
  • 相关文献

参考文献11

  • 1Kushibiki T, Nagata-kaajima N, Sugai M, et al. Targeting of Plas- mid DNA to renal interstitial fibroblasts by cationized gelatin [ J ]. Boil Pharm Bull, 2005,28(10) :2007-2010.
  • 2Mofidaira K, Morrissey J, Fitzgerald M, et al. ACE inhibition in- creases expression of the ETB receptor in kidneys of mice with uni- lateral obstruction [ J ]. Am J Physiol Renal Physiol, 2003,284 ( 1 ) :F209-217.
  • 3刘新晖,张锡庆,王晓东,张学兰,王科文,张亚,张德强,张希峰.兔肾微血管内皮细胞体外培养及其细胞表型的初步研究[J].中国微循环,2003,7(6):381-384. 被引量：6
  • 4Hocher B, Thone-Reineke C, Rohmeiss P, et al. Endothelin-1 transgenic mice develop glomerulosclerosis, interstitial fibrosis, and renal cysts but not hypertension [ J ]. J Clin Invest, 1997,99 (6) :1380-1389.
  • 5Aoki M, Yasutkae M, Murohara T. Derivation of functional endothe- lial progenitor cells from human umbilicia cord blood mononuclear ceils isolated by a novel cell filtration device [ J ]. Stem Cells, 2004,22(6) :994-1002.
  • 6Brodsky SV, Yamamoto T, Tada T, et al. Endothelial dysfunction in ischemic acute renal failure: rescue by transplanted endothelial cells[J]. Am J Physiol, 2002,282(6) :1140-1149.
  • 7Eddy AA. Progression in chronic kidney disease[ J]. Adv Chronic Kidney Dis, 2005,12(4) :353-365.
  • 8O' Connor DS, Sehechner JS, Adida C, et al. Control of paoptosis during angiogenesis by surviving expression in endothelial cells [J]. Am J phatol, 2000,256(2) :393-398.
  • 9Feng D, Nagy J, Hipp J, et al. Vesiculo-vacuolar orgenalles and the regulation of venule premeability to macormolceules by vaseular premebaility factor, histamine, and serotonin [ J ]. J Exp Med, 1996,183(5) :1981-1986.
  • 10Pepper MS, Fearrra N, Orei L,et al. Potent synergism between va- seular endothelial growth factor and basic fibroblast growth factor in the induction of angiogenesis in vitro[ J]. Biochem Biophys Res Commun, 1992,189(2) :824-831.

二级参考文献13

  • 1沈士亮,徐玉会.葡萄球菌A蛋白胶体金银法对血管内皮细胞第Ⅷ因子相关抗原的研究[J].中华病理学杂志,1989,18(1):69-70. 被引量：10
  • 2Ohashi R, Kitamura H,Yamanaka N.Peritubular capillary injury during the progression of experimental glomerulonephritis in rats.J Am Soc Nephrol,2000,11(1):47-56.
  • 3Kang DH,Joly AH,Oh SW,et al.Impaired angiogenesis in the remnant kidney model Ⅰ.Potential role of vascular endothelial growth factor and thrombospondin-1.J Am Soc Nephrol,2001,12(7):1434-1447.
  • 4Norman JT,Clark IM,Garcia PL.Hypoxia promotes fibrogenesis in human renal fibroblasts. Kidney Int,2000,58(6):2351-2366.
  • 5Masuda Y,Shimizu A, Mori T,et al.Vascular endothelial growth factor enhances glomerular capillary repair and accelerates resolution of experimentally induced glomerulonephritis.Am J Pathol,2001,159(2):599-608.
  • 6Kang DH, Kim YG, Andoh TF,et al.Post-cyclosporine-mediated hypertension and nephropathy: amelioration by vascular endothelial growth factor. Am J Physiol Renal Physiol,2001,280(4):727-736.
  • 7Tufro A,Norwood VF,Carey RM,et al.Vascular endothelial growth factor induces nephrogenesis and vasculogenesis. J Am Soc Nephrol,1999,10(10): 2125-2134.
  • 8Ostendorf T,Kunter U,Eitner F,et al.VEGF165 mediates glomerular endothelial repair.J Clin Invest,1999,104(7):913-923.
  • 9邹万忠.肾脏病理与临床第1版[M].长沙:湖南科学技术出版社,1993.58.
  • 10Ohashi R,Shimizu A,Masuda Y,et al.Peritubular capillary regression during the progression of experimental obstructive nephropathy. J Am Soc Nephrol,2002,13(7):1795-1805.

共引文献14

同被引文献54

  • 1庄峰,刘英莉,张薇,李宁丽.BMSCs移植对单侧输尿管结扎大鼠CD4^+CD25^+Treg的影响[J].上海交通大学学报（医学版）,2011,31(6):739-743. 被引量：4
  • 2孙岩,韩德恩,姜明山,韩福友.VEGF、ET-1在供体活细胞诱导长期存活的移植物中的表达[J].中华小儿外科杂志,2006,27(5):259-261. 被引量：1
  • 3Koyama Y,Maebara Y,Hayashi M,et al.Endothelinsreciprocally regulate VEGF-A and angiopoietin-1pro-duction in cultured rat astrocytes:Implications on as-trocytic proliferation[J].Glia,2012,60(12):1954-1963.
  • 4Li Q,Pan H,Guan L,et al.CITED2 mutation linkscongenital heart defects to dysregulationof the cardiacgene VEGF and PITX2Cexpression[J].Biochem Bio-phys Res Commun,2012,423(4):895-899.
  • 5Iwabe S,Lamas M,Vasquez Pelaez CG,et al.Aqueoushumor endothelin-1(Et-1),vascular endothelial growthfactor(VEGF)and cyclooxygenase-2(COX-2)levels inMexican glaucomatous patients.[J].Current Eye Re-search,2010,35(4):287-294.
  • 6Garrafa E,Caprara V,DiCastro V,et al.Endothelin-1cooperates with hypoxia to induce vascular-like struc-tures through vascular endothelial growth factor-C,-Dand-A in lymphatic endothelial cells[J].Life sciences,2012,91(13/14):638-643.
  • 7Koyama,Y.,Maebara,Y.,Hayashi,M.et al.Endo-thelins reciprocally regulate VEGF-A and angiopoietin-1production in cultured rat astrocytes:Implications onastrocytic proliferation[J].Glia,2012,60(12):1954-1963.
  • 8陈香美;刘志红.肾小球肾炎遗传研究新进展[A],2011196-197.
  • 9Sun D,Wang Y,Liu C. Effects of nitric oxide on renal interstitial fibrosis in rats with unilateral ureteral obstruction[J].{H}Life Sciences,2012,(23):900-902.
  • 10Rojas A,Chang FC,Lin SL. The role played by perivascular cells in kidney interstitial injury[J].{H}Clinical Nephrology,2012,(05):400-408.

引证文献4

二级引证文献33

山东医药
2012年 第10期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部