黄梁木组培快繁技术研究

Study on Tissue Culture of Neolamarckia cadamba

对黄梁木组培快繁及规模化育苗技术进行了研究,结果表明:最适诱导培养基为MS+6-BA 1.0 mg/L+IBA0.1 mg/L+GA3 2.0 mg/L;最佳增殖培养基为MS+6-BA 1.0 mg/L+IBA 0.05 mg/L,增殖系数达3.4以上;适宜的生根培养基为MS+NAA 0.1 mg/L,15 d内生根率100%以上,平均根数为7.8条,平均根长为1.42 cm.

Tissue culture techniques and large scale micropropagation of Neolamarckia cadamba were studied in this paper.The results showed that the optimal inducing medium was MS＋6-BA 1.0 mg/L＋IBA 0.1 mg/L＋GA3 2.0 mg/L;the optimal multiplication medium was MS ＋6-BA 1.0 mg/L ＋ IBA 0.05 mg/L,the highest multiplication rate was 3.4.The optimal rooting medium was MS＋NAA 0.1 mg/L.Within 15 d,the rooting rate could reach 100%,the average number of roots was 7.8,and the average length of roots was 1.42 cm.