姜黄素对体外培养的人食管癌Ec-109细胞增殖和凋亡的影响

EFFECTS OF CURCUMIN ON THE PROLIFERATION AND APOPTOSIS OF HUMAN ESOPHAGEAL CARCINOMA Ec-109 CELLS IN VITRO

目的研究姜黄素对体外培养的人食管癌(esophageal carcinoma 109,Ec-109)细胞增殖和凋亡的影响。方法采用噻唑兰(methyl thiazolyl tetrazolium,MTT)法检测不同浓度姜黄素对人食管癌Ec-109细胞增殖的影响;流式细胞术检测Ec-109细胞的凋亡情况;免疫细胞化学方法检测Fas、增殖细胞核抗原(proliferating cellnuclear antigen,PCNA)蛋白表达的情况。结果姜黄素可显著抑制Ec-109细胞的增殖,呈剂量和时间依赖性,半数抑制浓度(inhibitory concentraltion 50,IC_(50))为22.09μmol/L(姜黄素浓度为80μmol/L作用60h)。不同浓度姜黄素均可诱导Ec-109细胞凋亡。姜黄素可显著下调Ec-109细胞PCNA蛋白表达,显著上调Fas蛋白表达。结论姜黄素可抑制体外培养的人食管癌Ec-109细胞的增殖,诱导其凋亡。

Objective To observe the effects of curcumin on the proliferation and apoptosis of human esophageal carcinoma 109 （ Ec - 109 ） cell line in vitro. Methods The inhibitory effect of curcumin on the proliferation of human Ec - 109 cells at different concentrations was determined by methyl thiazolyl tetrazolium （MTY）assay. The changes of cell apoptosis rate of tumo：r cells were determined by flow cytometry （FCM）. The expressions of Fas and proliferating cell nuclear antigen（PCNA） proteins were examined by immunocytochemical technique. Results Curcumin significantly inhibited the proliferation of Ec -109 cells in a dose -and time -dependent manner,with the inhibitory concentrahion 50（ IC50 ） of 22.09μmol/L（ Curcumin concentration for 80μmoL/L role of 60h）. Different concentrations of curcumin can induce apoptosis of Ec - 109 cell. Curcumin dramatically inhibited protein expression of PCNA, while upregulated the expression of Fas protein. Conclusion Curcumin can inhibit the proliferation of Ec - 109 cells and induce the apoptosis of Ee - 109 cells in vitro.