摘要
目的研究臭氧预处理对于脂多糖诱导的急性肾功能衰竭(ARF)时水通道蛋白2(AQP-2)的影响。方法 30只雄性Wistar大鼠随机分成3组:空白对照组(CL组)、内毒素处理组(LP组)、臭氧预处理组(OP组)。OP组每天在同一时间腹腔注射1次臭氧(1mg/kg,浓度50μg/mL),每次约4.4～5mL,连续10d。LP组每天注射相同体积的空气。LP组、OP组于最后1次预处理后24h腹腔注射脂多糖10mg/kg。脂多糖注射4h后处死大鼠留取肾脏标本,免疫组织化学染色观察AQP-2表达及肾脏组织核转录因子(NF)-κB的活化程度。结果与CL相比,LP组与OP组AQP-2表达均下降(P<0.01),但OP组下降低于LP组(P<0.01)。CL组NF-κB的活化极少,而LP组与OP组NF-κB活化程度均增高(P<0.01),但LP组升高较OP组更为明显(P<0.01)。结论臭氧预处理可以抑制肾脏AQP-2的下降,减轻肾脏组织的损伤。其机制与臭氧抑制NF-κB的活性,调节炎症的产生和消退,并且保护细胞的氧化还原状态有关。
Objective To analyze the effect of ozone oxidative preconditioning on the expression of AQP-2 in LPS-induced acute renal failure（ARF）.Methods Thirty health male Wistar rats were evenly randomized into 3 groups： control group（CL）,LPS group（LP） and ozone oxidative preconditioning group（OP）.OP group was administered gaseous ozone intraperitoneally 1 mg/kg for ten consecutive days in the same time,one per day 4.4-5.0 mL with ozone concentration of 50 μg/mL.LP group was administered with the same volume of air intraperitoneally.After 24 hours of the last ozone treatment,LP and OP received LPS 10 mg/kg by intraperitoneal administration.The animals were sacrificed 4 hours after LPS injection.Immunohistochemical method was used to determine the expression of AQP-2 and the activity of NF-κB in the nephridial tissues.Results Compared with the CL group,the expression of AQP-2 was apparently lower in the LP group and OP group,but the expression of AQP-2 in the OP group was higher than that in the LP group（P0.01）.The CL group has quite a low activity of NF-κB compared with that in the LP group and OP group（P0.01）.But the NF-κB level was less significantly increased in the OP group than that in the LP group（P0.01）.Conclusion Ozone oxidative preconditioning inhibits the downregulation of AQP-2 and ameliorate injuries in nephridial tissues.The mechanism may be related to its preservation of cell redox state and inhibition of NF-κB which regulates the induction and resolution of inflammation.
基金
国家自然科学基金(81071530)
