摘要
:[目的]探索α 双炔失碳酯对人原发卵巢癌组织的作用及其与E2 水平、ER表达之间的关系。[方法]应用MTT法检测α 双炔失碳酯对19例人原发卵巢癌组织的生长调节作用 ;采用免疫组化ABC法测定19例石蜡组织ER含量 ;术前抽取静脉血 ,酶标法测E2 水平。[结果](1)100μmol/Lα 双炔失碳酯作用72小时后 ,肿瘤细胞生长抑制率大于30%者共7例(敏感组) ,占36 8% ;生长抑制率小于30%者共12例(不敏感组) ,该抑制作用与病人年龄、绝经与否、临床期别、分化程度、病理类型等无明显相关。(2)敏感组E2 的平均水平为(45 07±46 84)pg/ml,不敏感组E2 的平均水平(25 77±26 41)pg/ml(P>0 05)。(3)ER阴性共7例 ,抑制率大于30%者占14 3%(1/7例) ,ER阳性共12例 ,抑制率大于30%占50%(6/12例)(P>0 05)。[结论]α 双炔失碳酯对原发卵巢癌细胞有一定抑制作用。ER阳性 ,E2 水平高的患者对α 双炔失碳酯的敏感性有高于ER阴性 ,E2
To study the effect of α Anordrin on primary ovarian cancer tissue and the relationship between α Anordin and ER expression,E2 level The inhibitory effect of α Anordrin on tissues of 19 cases with primary ovarian cancer were measured by MTT methods ER expression of cancer tissues and E2 levels of blood samples were detected by immunohistochemistry and enzyimmunoassay respectively (1)72 hours after treatment of 100μmol/L α Anordrin,cancer cells inhibited significantly(Inhibitory rate >30%) in 7cases(36 8%) as sensitive group The other 12 cases being inhibited less than 30% as insensitive group (2)E2 levels in the sensitive and insensitive group were (45 07±46 84)pg/ml,and(25 77±26 41)pg/ml respectively(P>0 05) (3)Only 1 case was inhibited more than 30% in 7 ER negative cases Inhibit rate>30% occurred in 6 of 12 ER positive cases(P>0 05) [Conclusion]α Anordrin might have inhibitory effect on primary ovarian cancer
