白色念珠菌肠内定植导致局部调节因子表达增加

Colonization of Candida albicans in Intestine Inducing Increased Expression of Cytokine in Gut Associated Lymph Tissue

观察白色念珠菌肠腔内定植后局部调节因子表达变化。方法 采用无特殊病原菌小鼠定植白色念珠菌 ,分别于定植后 3 d,7d,14d活杀 ,观察如下指标 :盲肠内白色念珠菌总数 ;潘氏结 (PP)中斑点杂交观测 IL -4,IL - 6表达变化 ;通过原位杂交反映固有层中 (L P) IL - 6表达变化 ;EL ISA测定肠匀浆中 IL - 6水平。结果 白色念珠菌总数以定植 3 d,7d较高 ,14d则明显下降 ;PP中 IL - 4表达以定植 14d最高 ;L P中 IL - 6阳性细胞数增加以定植 7d最高。结论 肠内白色念珠菌定植能引起局部 IL - 4,IL - 6表达增加。

OBJECTIVE To investigate the change in IL-4 and IL-6 expression in local intestinal mucosal immunity tissue during colonization of Candida albicans.METHODS The total number of C.albicans in intestine was calculated by culture;IL- expression in PP was observed by spot hybridization,meanwhile IL-6 expression in LP was observed by in situ hybridization. RESULTS The changes in total number of Candida were obvious after inoculation.At PCD 3 and PCD 7 ,the number of candidas was higher,but it decreased at PCD 14 .At PCD 14 ,IL-4 expression in PP was the highest, while at PCD 7 IL-6 expression in LP was the top one.CONCLUSIONS Local increasing in IL-4 and IL-6 expression may be induced by colonization of C.albicans.