我国首次发现的HIV-2型病毒的分子生物学证据

Molecular Evidence of the First Case of HIV-2 Infection in China

[目的 ]从分子生物学角度进一步证实我们发现的 1例 HIV感染者为国内首例 HIV- 2型病毒携带者。 [方法 ]从 Genebank查询已知的 HIV- 2核酸序列 ,通过序列比较以及酶切位点分析查找 HIV- 2基因组中保守区 ,应用套式PCR扩增出 HIV- 2基因组 L TR- GAG区的～ 330 bp的核酸片段 ,并经限制性酶切位点和序列分析证实该扩增片段为HIV- 2特异性片段。 [结果 ]实验表明 ,扩增片段为 HIV- 2特异性片段 ,序列分析也表明感染者体内病毒为 HIV- 2。 [结论 ]从遗传学上进一步支持该感染者为 HIV- 2携带者。

Objective] To further confirm the first case infected with HIV-2 in China. [Methods] DNA extracted from PBMC of the HIV-2 infection was amplified with the primers located at LTR-GAG region of HIV-2 genome. PCR product was analyzed by HaeIII digestion and sequencing. [Results] The length of the PCR product was 330bp and contained the specific segment of HIV 2 in the region. Sequence analysis also showed the virus being HIV-2. [Conclusions] The study supports fully our finding as described previously in china.