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胡椒碱对H_2O_2所致兔心房肌细胞瞬时外向钾电流异常的保护作用

Protective effect of piperine on H_2O_2-induced abnormal transient outward potassium current in rabbit atrial myocytes
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摘要 目的研究胡椒碱对H2O2引起的兔单个心房肌细胞瞬时外向钾电流(Ito)异常的保护作用。方法采用全细胞膜片钳技术分析50μmol/L的H2O2对兔单个心房肌细胞Ito的影响,并研究预先应用7μmol/L的胡椒碱对其的保护作用。结果 7μmol/L胡椒碱对正常兔心房肌细胞Ito及其通道动力学无显著影响。在50μmol/L H2O2作用下,兔心房肌细胞Ito峰值由(39.3±5.4)pA/pF降低至(32.8±2.0)pA/pF(P<0.05),电流-电压曲线下移,通道稳态激活曲线右移,通道稳态失活曲线及恢复时间不变,关闭态失活加速。预先给予7μmol/L胡椒碱,明显减轻H2O2对Ito的抑制作用(P<0.01),并可减轻H2O2对瞬时外向钾通道动力学的异常影响。结论胡椒碱可减轻氧化应激对心房肌细胞Ito的影响。 Objective To study the protective effect of piperine on H2O2-induced abnormal transient outward potassium current(Ito) in single rabbit atrial myocyte. Methods Effect of H202(50 μ mol/L) and piperine(7 μ mol/L) on Ito in single rabbit atrial myocyte was analyzed using the whole cell patch clamp technique. Results Piperine(7 μ mol/L) had no significant effect on Ito and its transient outward potassium channel dynamics. The peak value of Ito in rabbit atrial myocytes decreased to (32.8 ± 2.0)pA/pF from (39.3 ± 5.4) pA/pF(P〈0.05), the current-voltage curve of Ito was shifted down, the channel steady-state activation curve of Ito was shifted to the right, the steady-state inactivation curve and the recovery time of Ito from inactivation did not change, the closed-state inactivation of Ito was accelerated, when H2O2(50 μ mol/L) was applied. Piperine(7 μ mol/L) significantly alleviated the inhibitory effect of H2O2 on Ito and its transient outward potassium channel dynamics(P〈0.01). Conclusion Piperine can alleviate H2O2-induced abnormal Ito in rabbit atrial mvocvtes.
出处 《军医进修学院学报》 CAS 2012年第5期496-499,共4页 Academic Journal of Pla Postgraduate Medical School
基金 国家自然科学基金项目(30772886)~~
关键词 胡椒碱 过氧化氢 瞬时外向钾电流 piperine hydrogen peroxide transient outward potassium current
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