LRP16影响Hela细胞对电离辐射的敏感性

Effect of LRP16 on sensitivity of Hela cells to ionizing radiation

目的探索LRP16的表达对Hela细胞的电离辐射敏感性是否产生影响。方法将真核表达质粒(pcDNA-3.1和pcDNA-3.1-LRP16)以及抑制LRP16表达的小干扰RNA:control siRNA、siRNA-374分别转染入Hela细胞内,并通过电离辐射处理,导致DNA损伤。采用单细胞凝胶电泳法(SCGE)检测转染了质粒细胞的DNA损伤程度;CCK-8试剂盒检测细胞活力;流式细胞技术测定抑制LRP16的表达对Hela细胞凋亡率的影响。结果电离辐射后,与对照组相比,转染了LRP16的Hela细胞损伤修复能力增高(P<0.05),而且细胞凋亡率明显降低(P<0.05)。抑制LRP16的表达后,Hela细胞的细胞活力比对照组明显降低(P<0.05)。结论 LRP16蛋白能够降低Hela细胞对电离辐射的敏感性。

Objective To study the effect of LRP16 expression on sensitivity of Hela cells to ionizing radiation （IR）. Methods Hela cells were transfected with Eukaryon-expressing plasmids（pcDNA-3.1, pcDNA-3.1-LRP16）, and siRNA, control siRNA, siRNA- 374 inhibiting expression of LRP16, and underwent IR to induce DNA damage. DNA damage in plasmid-transfected Hela cells was detected by single cell gel electrophoresis（SCGE）. Viability of Hela cells was assayed with a CCK-8 kit. Effect of inhibiting LRP16 expression on apoptosis of Hela cells was examined by flow cytometry. Results The viability of Hela cells was significantly lower after transfection with plasmid than before transfection with plasmid（P〈0.05）. After treated with etoposide, the number of apoptotic cells was significantly greater in experimental group than in control group（P〈0.05）. Conclusion Over-expression of LRP16 can increase the sensitivity of Hela cells to IR and promote their proliferation.