柴蔻冲剂对大鼠慢性酒精性肝损伤防护作用的实验研究

EXPERIMENTAL STUDY ON RAT'S CHRONIC ALCOHOLIC LIVER INJURY PREVENTED WITH CHAIKOU GRANULE

目的：观察中药复方柴蔻冲剂对大鼠慢性酒精性肝损伤的防护作用。方法：来用高浓度酒精每ｄ分次 灌胃，同时酒精饮料饲养制备大鼠慢性酒精肝损伤模型，应用光镜和电镜观察大鼠肝脏组织学变化及超微结 构，检测血清ＡＬＴ、ＡＳＴ、ＧＣＴ活性。观察不同剂量柴蔻冲剂对酒精中毒性肝损伤的保护作用。结果：酒精损伤 组肝功能有严重损伤，所有大鼠均出现了脂肪肝，９０％的动物发生了酒精性肝炎，部分大鼠（２２％）可见肝间质 反应性增生，柴蔻冲剂预防组肝功能、肝组织学及超微结构损伤显著减轻，优于丙基硫氧嘧啶对照组。结论：柴 蔻冲剂对酒精性肝损伤具有良好的防护作用。

Aim: To observe the pretective effect of ChaiKou granule on rat from the chronic alcoholic liver injury. Methods:The model with chronic lcoholic liver injury was replicated by means of astrolavaging high concentration alcohol several times a day, as well as feeding alcoholic beverage,meanwhile observing the liver's histology and microstructure by light and electronic changes of microscope, and assaying the activity of ALT,AST and GGT to assess the liver function.The effect of different does Chaikou granule was evaluated respectively on its prevention from the alcoholic liver injury. Results:The animal model was successfully replicated,which shoed sever live dysfunction, with fatty liver in all rats, alcoholic hepatitis was 90 percent and reactive hepatic interstitial proliferation was 22 percent. Compared with Essentiale group, less liver injury was observed in different level such as live function, histology & microstructure among the group with ChaiKou granule administrated. Conclusion: The ChaiKou granule was proved to have great efficiency to prevent rat from the alcoholic liver injury.