全脑缺血-再灌注损伤后炎性细胞因子的变化及其意义

The changes in pro-inflammatory cytokines in brain and plasma in rats with complete brain ischemia-reperfusion injury and their significance

目的 :探讨炎性细胞因子〔肿瘤坏死因子 α(TNFα) ,白细胞介素 1β(IL 1β)和白细胞介素 8(IL 8)〕在全脑缺血再灌注损伤中的作用。方法 :采用大鼠全脑缺血再灌注模型 ,动态测定脑组织及血浆中TNFα、IL 1β及 IL 8含量变化。结果 :脑组织中 TNFα、IL 1β及 IL 8含量相继释放增加 ,并持续至再灌注后第 5日仍维持较高水平 ,上述细胞因子的血浆变化滞后于脑组织的变化。结论 :炎性细胞因子参与了全脑缺血再灌注损伤的发生和发展过程 ;干预这些炎性细胞因子将会减轻脑组织缺血再灌注损伤的急性炎症反应 。

Objective: To explore the role of inflammatory cytokines in the complete brain ischemiareperfusion injury.Methods:Using a rat model of complete brain ischemiareperfusion injury with fourvessel occlusion,the changes in levels of tumor necrosis factorα(TNFα),interleukin1β(IL1β) and IL8 in brain tissues and in plasma were dynamically observed.Results:The levels of TNFα,IL1β and IL8 in brain tissues were sequentially increased and maintained at high levels until 5 days after complete brain ischemiareperfusion.The alterations in aforementioned cytokines in plasma lagged behind those in the brain tissues.Conclusions:The proinflammatory cytokines may participate in the onset and development of brain injury after complete brain ischemiareperfusion.